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Kardasewitch Method
Methods of removing formalin pigment

Lillies method
Methods of Removing formalin pigment

picric acid method
Methods of Removing formalin pigment

10% Formol Saline
Aldehyde Fixatives

10% Neutral Buffered Formalin
Aldehyde Fixatives

Formol-corrosive (Formol-Sublimate)/Formol Mercuric Chloride
Aldehyde Fixatives

Glutaraldehyde
Aldehyde Fixatives

Mercuric Chloride
Metallic Fixatives

zenkers fluid
Metallic Fixatives (Mercuric Chloride Based)

zenkers formol (helly’s solution)
Metallic Fixatives (Mercuric Chloride Based)

Heidenhains susa solution
Metallic Fixatives (Mercuric Chloride Based)

Chromic acid (1-2% aqueous)
Chromate Fixatives

Potassium dichromate (3% aqueous)
Chromate Fixatives

Regaud’s (Moller’s) Fluid
Chromate Fixatives

Orth’s Fluid
Chromate Fixatives

Lead Fixatives

Picric Acid

Bouin’s solution
Picric acid fixatives

Brasils alcoholic picroformol fixative
Picric acid fixatives

Glacial acetic acid

Alcohol fixatives

Methyl Alcohol (100%)
Alcohol Fixatives

Ethyl Alcohol (70-100%)
Alcohol Fixatives

Carnoy’s Fluid
Alcohol Fixatives

Alcoholic Formalin (Gendre’s Fixative)
Alcohol Fixatives

Newcomers Fluid
Alcohol Fixatives

Osmium tetroxide

Flemmings solution
Osmium tetroxide

Flemmings without acetic acid
Osmium tetroxide

Trichloroacetic acid

Acetone

Heat fixation

Secondary fixation

post chromatization

washing out

factors affecting fixation

General principles & Precautions
READ PLEASE

Effects of fixatives in general

Microanatomical fixatives
A. According to ACTION

Cytological fixatives

Nuclear fixatives
Cytological Fixatives

Cytoplasmic Fixatives
Cytological Fixatives

histochemical fixatives
