Microbiology: cloning

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6 Terms

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Describe what The PCR is

Polymerase Chain Reaction: synthesizes large quantities of a DNA fragment

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Three repeated steps of the PCR

Denaturing, Annealing, and extension

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Describe Denaturing

target DNA is denatured with heat

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Describe annealing

Primers bind to target DNA

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Describe extension

Copies of target DNA are synthsized

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What to know about Restriction Endonucleases

These enzymes recognize a specific sequence and cut it