8. Sperm Count (Haemocytometrical, Photocolormetrical Methods)

How can the concentration of spermatozoa be determined?

1. Direct cell count method - haemocytometrical method using a counting chamber (Burker's chamber).
2. Nephelometric method using a nephelometer.
3. Electronic particle counter.
4. Density-standard method.
5. Cell-volume method.

How does the haemocytometrical method (direct cell count method) work?

In the haemocytometrical method, a semen sample is mixed and diluted to a known ratio. The diluted sample is then introduced into a counting chamber, and the number of spermatozoa is counted within a specified area. The concentration of cells in a known volume of semen is calculated based on the count obtained and the diluted ratio.

What is the Nephlometric method for determining sperm concentration?

In the nephelometric method, the semen sample is carefully mixed and blown into a test tube or container with a clear diluent. The mixture is then measured for optical density using a nephelometer. The instrument must be calibrated against diluted semen of known concentration.

What are some other methods for determining sperm concentration?

1. electronic particle counter
2. density-standard method
3. cell-volume method