AMS-Based Detection of Oxidation

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5 Terms

1
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What is AMS?

A midsize reagent that binds reduced cysteines

2
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What is the first step of AMS detection?

Protein extraction under acidic conditions

3
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What does AMS binding cause on SDS-PAGE?

Mobility shift

4
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What is a limitation of AMS detection?

Type of oxidation is not revealed

5
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Why are antibodies needed?

To detect specific proteins