Chymotrypsin mechanism

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Last updated 6:16 PM on 3/20/26
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7 Terms

1
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Step #1

SUBSTRATE BINDING: The peptide side chain fits into a hydrophobic pocket, aligning the peptide bond for attack

<p>SUBSTRATE BINDING: The peptide side chain fits into a hydrophobic pocket, aligning the peptide bond for attack</p>
2
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Step #2

NUCLEOPHILIC GENERATION: The Ser and His residue interaction create a Ser alkoxide, this nucleophile attacks the carbonyl carbon forming a tetrahedral acyl-enzyme intermediate that is stabilized bythe oxyanion hole

<p>NUCLEOPHILIC GENERATION: The Ser and His residue interaction create a Ser alkoxide, this nucleophile attacks the carbonyl carbon forming a tetrahedral acyl-enzyme intermediate that is stabilized bythe oxyanion hole</p>
3
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Step #3

ACYLATION: Collapse of the tetrahedral intermediate reformsthe carbonyl, releasing the amino terminal fragment and leaving an acyl Ser-95 ester

<p>ACYLATION: Collapse of the tetrahedral intermediate reformsthe carbonyl, releasing the amino terminal fragment and leaving an acyl Ser-95 ester</p>
4
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Step #4

WATER ACTIVATION: A water molecule is deprotonated by the catalytic base that produces a hydroxide ion

<p>WATER ACTIVATION: A water molecule is deprotonated by the catalytic base that produces a hydroxide ion</p>
5
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Step #5

DEACYLATION:The hydroxide attacks the acyl-Ser ester creating a 2nd tetrahedral intermediate that is stabilized in the oxyanion hole.

<p>DEACYLATION:The hydroxide attacks the acyl-Ser ester creating a 2nd tetrahedral intermediate that is stabilized in the oxyanion hole. </p>
6
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Step #6

BREAK-OFF FROM THE ENZYME: The intermediate collapses, releasinf the carboxyl terminal fragment andrestoring the free Ser, reasy for another catalytic round

<p>BREAK-OFF FROM THE ENZYME: The intermediate collapses, releasinf the carboxyl terminal fragment andrestoring the free Ser, reasy for another catalytic round</p>
7
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Step #7

PRODUCT DISSOCIATES:The product dissocates an the process starts over with a new substrate

<p>PRODUCT DISSOCIATES:The product dissocates an the process starts over with a new substrate</p>