Sree Marella - Chapter 5 : Bacterial Transformation and PCR

Ahmed - 7th

What is the clinical significance of bacterial transformation in biotechnology?

allows production of insulin and vaccine development

When did Griffith discover transformation?

1928

How did the mouse die in station 1

an S strain bacteria was injected

what did the s strain contain

live bacteria w capsule

how was the r strain different from the s strain

r strain bacteria did not contain capsule

what happened in station 3 of Griffith’s experiment?

heat treated s strain bacteria was

what happened in station 4

mixed strain was injected, mouse dies

how did the bacteria demonstrate genetic variation

R strain was able to cause disease like s strain

what happens during DNA release and uptake in the transfer process

Recipient (-) becomes donor (+)

what happens during DNA integration

DNA segment gets inserted into cell’s genome

what happens during bacterial division in transformation

newly acquired plasmid is passed onto daughter cells

what is the clinical significance of bacterial conjugation

what are different bacterial mating types

what is the fertility factor

what is the conjugation bridge

how are genes transferred during conjugation

what is high frequency recombination

what is bacterial transduction

what happens during bacteriophage attachment

what happens during phage DNA replication

what happens during packaging and phage release

what are the steps of generalized transduction

what happens during prophage integration and excision

what is the hybrid DNA formation

how is DNA transferred to recipient

what is pre-integration state

what is the post-integration state

what is the clinical significance of bacterial transduction