BMSC 240 Laboratory Techniques Lecture 15: Module 7: Protein Electrophoresis

These flashcards cover key concepts from protein electrophoresis and chromatography techniques discussed in the lecture.

Electrophoresis

The migration of ions in an electric field, where the velocity of ions is proportional to their charge and inversely proportional to their size and shape.

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis; a method to denature proteins and impart a negative charge using SDS.

Acrylamide

A monomer used to create gels for electrophoresis, known for its neurotoxic, carcinogenic, and teratogenic properties.

Polymerization

The process of combining small molecules (monomers) to form a larger, more complex molecule (polymer), as in the creation of gel matrices.

Rf (Relative Mobility)

A ratio used to compare how far a protein band moves in comparison to the front of the dye during electrophoresis.

Stacking Gel

The top layer of a two-layer gel system used in electrophoresis that allows proteins to concentrate before entering the running gel.

Running Gel

The bottom layer of a gel used in electrophoresis where the actual separation of proteins based on size occurs.