MICRO LAB QUIZ 1 PART 2

name the 3 substrates are used in the decarboxylase reaction

lysine, ornithine, arginine

how does the decarboxylase enzyme modify the substrate?

removes acid group from amino acids- makes more alkaline

name the specific enzyme for each of these decarboxylase reactions- lysine

decarboxylates lysine

name the specific enzyme for each of these decarboxylase reactions- arginine

decarboxtlates arginine

name the specific enzyme for each of these decarboxylase reactions- ornithine

decarboxylates ornithine

why is mineral oil used in the decarboxylase reaction?

creates an anaerobic environment

what is the ph indicator in the decarboxylase reaction

bromocresol purple

what color is a positive response for the decarboxylase reaction

purple color

name a bacterium GENUS that is positive for each of these decarboxylase reactions- ✔ Lysine decarboxylase (+)

e.coli

name a bacterium GENUS that is positive for each of these decarboxylase reactions-Ornithine decarboxylase

enterobacter

name a bacterium GENUS that is positive for each of these decarboxylase reactions-Arginine decarboxylase

citrobacter

what is the substrate for the phenylalanine deaminase reaction

phenylalanine

what enzyme does the phenylalanine deaminase reaction test for?

phenylalanine deaminase

how does the phenylalanine deaminase enzyme modify the substrate?

removes the amino group from phenylalanine and converts to products

what are the 2 products of the phenylalanine deaminase reaction?

phenylpyruvate and ammonia

what reagent is used to test for the products of the phenylalanine deaminase reaction?

ferric chloride

what PRODUCT does the reagent specifically test for the presence of?

phenyl pyruvate

what color is a positive result for the phenylalanine deaminase reaction?

green color

which bacterium GENUS is positive for the phenylalanine deaminase reaction

proteus