DNA Replication in E. coli

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Practice flashcards for the topic of DNA replication, focusing on key concepts, enzymes, and processes involved.

Last updated 1:07 AM on 4/2/25
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10 Terms

1
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What is the structure that forms during DNA replication where the DNA unwinds and separates?

Replication fork.

2
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What is the function of DNA polymerase (DNA pol III) in DNA replication?

It synthesizes new DNA strands by adding nucleotides complementary to the template strand.

3
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What are Okazaki fragments?

Short segments of DNA synthesized on the lagging strand during DNA replication.

4
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What enzyme synthesizes the RNA primer needed for DNA replication?

Primase.

5
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What are the two types of strands being synthesized during DNA replication?

Leading strand and lagging strand.

6
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What is the role of DNA ligase in DNA replication?

It joins together Okazaki fragments and links newly synthesized DNA.

7
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What is formed at the end of DNA replication?

Two identical DNA molecules.

8
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Which bacterium is used as a model organism in studying DNA replication?

E. coli.

9
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What is the direction of synthesis for the leading strand?

5' to 3' direction.

10
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What happens to RNA during DNA replication when DNA polymerase I is involved?

DNA polymerase I removes RNA primers and replaces them with DNA.