MCBLab \\ Restrcition Digest

restriction enzymes

recognize and cut specific sequences of DNA

5uL of gel star

dye that binds to DNA to see it

4uL of tracking dye

dye that visualizes the movement of dna

vectors

carry gentic material to be used to target cells

plasmid

small circular DNA found in bacterial cells

ligation

joining of 2 amino acids

In-vivo

studying inside an organism

In vitro

stuyding outside an organism

recombinant DNA

combines DNA from different sources to create new genetic combinations

resctrion enzymes (defensive mechinism for dna)

they are proteins that cut DNA a specfic recongnition sites

resctriction mapping

identifys and maps location of resctrion sites in a DNA molecule

Examples of Restriction Enzymes ?

BamHI, EcoRI, HindIII, Pst1

how do you describe name resctrion enzymes?

B—→genus

am—→ species

H—→ strain

1—→ number of enzyme discovered

cloning

inserts DNA fragments into vectors

Blunt ends

straight cuts of DNA

Sticky ends

staggered cuts of DNA

gel electrophoresis

sorts DNA fragments based on their lengths

During gel electrophoresis Dna move from negative to positive t/f?

true

whats the total plasmid size

4,539bps

the more lighter the molecule ?

the more faster it moves.

the more heavier the molecule ?

the slower it moves.

cathode (-) ——→ anode (+)? true or false

true? C.N.A.P