Lab Techniques

____________ separates macromolecules (Proteins, DNA, RNA) using an electrical charge

Gel Electrophoresis

Explain “An Ox, Red Cat”

Anode is used for Oxidation, Cathode is used for Reduction

During Gel Electrophoresis, electrons are transferred from the _________ to the __________

Anode to the Cathode

During Gel Electrophoresis, the anode becomes __________ charged, and the cathode becomes ____________ charged

Anode becomes (+) charged, Cathode becomes (-) charged

In Gel Electrophoresis, molecules will separate based on ________ and _________

Size and Charge

Gel Electrophoresis

• The gel creates ____________, causing larger molecules to have a harder time moving down the gel

Resistance

Gel Electrophoresis

• ___________ charged molecules will move towards (+) anode (down)

Negatively

Gel Electrophoresis

• _____________ molecules will move the furthest down the gel

(-) smaller

Gel Electrophoresis, Native PAGE

• ____________ Conditions

Non-Denaturing

Gel Electrophoresis, Native PAGE

• Separates proteins based on _________ alone (maintain structure)

Size

Gel Electrophoresis, SDS PAGE

• ____________ Conditions, but sulfide bonds remain in tact

Denaturing

Gel Electrophoresis, SDS PAGE

• Helps identify _____________

Multiple Subunits

Gel Electrophoresis, SDS PAGE

• Separate proteins based on _________ alone

Mass

Gel Electrophoresis, Reducing SDS PAGE

• ____________ Conditions

Completely Denatured

Gel Electrophoresis, Reducing SDS PAGE

• Reduces ____________

Disulfide Bonds

__________ bonds form as a result of 2 Cysteine’s becoming oxidized

Disulfide

Gel Electrophoresis, Isoelectric Focusing

• ____________ with _______ on one side and ________ on the other

pH Gradient, Low to High

Gel Electrophoresis, Isoelectric Focusing

• Proteins travel until they reach their ________

pI