Lab Exam Study Game -(Foley edited)

High lactose fermentor.

If an organism grows a green, metallic culture on an EMB streak plate then it is likely a...

FTM

Resazurin is an indicator that is used in which media?

Zone of Inhibition

The region surrounding an antibiotic susceptibility test is known as the [________].

The capsules do not get stained, the background and cell are stained.

When performing a capsule stain what color are the capsules stained?

flagella (Euglena has a flagella but Paramecium has cilia)

Euglena can be differentiated from paramecium by looking for their [________].

Complete color change indicates high susceptibility to forming dental caries.

If a snyder test turns completely yellow what does this signify?

Beta hemolysis

Which type of hemolysis is complete hemolysis?

Rehydrated rabbit plasma

What is the medium used in the coagulase test made of?

Lysine decarboxylase positive

If Lysine Iron Agar test results in media with purple

Antibody.

An indirect ELISA is used for determining total [________] concentration.

Psychrophile.

An organism that is well adapted to cold environments is known as a [________].

Cysteine desulfurase or Thiosulfate reductase

In the SIM test a black precipitate is a positive indicator for which enzyme?

Kovac's reagent

Which indicator is used in SIM to test for indole production?

You take the diameter (in mm).

When measuring the zone of inhibition of an antibiotic where do you measure?

Durham

A [________] tube is sometimes used to test for gas production.

2H₂ + O₂ --Palladium catalyst→ 2H₂O

What is the equation for the reaction that goes on in the anaerobic jar?

When using anything other than the scanning lens.

When is it not okay to use the coarse focus on a microscope?

Negative Stains & Capsule

What are the two stains that we do not heat fix in this lab?

Iodine.(helps crystal violet stay in cell wall)

What is the mordant used in a gram stain?

Trichomonas vaginalis (STD)

Which organism causes Trichomoniasis?

Denitrifier

If an organism produces gas during the nitrate test it is considered to be a [________].

The oxygen requirements of an organism.

The anaerobic jar is used to determine what?

Staphylococcus aureus

Which organism results in a color change to the MSA plate (red/oink to yellow)?

Pink

What is the color of a positive Urease test?

The conversion of urea to ammonia which raises pH

What causes a positive Urea test to change color?

7.5%

What is the concentration of salt on an MSA plate?

False.

Organisms that exhibit brownian movement likely have flagella or cilia. (True/False)

Purple.

A gram positive organism will appear which color under microscope? (after gram staining)

10x

The optical lenses on our microscope has a magnification of [________].

90% (1 log value)

D-Value is the time needed to reduce the number of viable organisms in a given population by [?]%.

Staphylococcus

MSA plates are a selective media that select for members of which genus?

Incomplete

The greenish zone around the colonies of an alpha-hemolytic organism is a result of [________] lysis.

2,3-butanediol

A positive result of the Voges-Proskauer test indicates an organism is capable of [________] fermentation.

Bubbles of oxygen gas

A positive result of the Catalase test will result in the production of [________].

Resazurin

What is the color indicator used in the FTM test?

Shape & size. (more accurate because no heat fix)

A negative stain is helpful to determine [________].

Mycobacterium

What is the primary genus of bacteria that we use the acid fast stain to identify?

Pinkish/purple.

What color do acid fast bacteria appear following an acid fast stain?

Strict aerobe.

If an organism does not grow while in the anaerobic jar it is a [________].

To sharpen the image-same refractive index as glass- no loss of light

What is the purpose of immersion oil?

Immunochromatography

A pregnancy test is an example of what test performed in lab?

Colony-forming Unit

What does CFU stand for?

plaque-forming unit

What does PFU stand for?

Bacteriophage

What is a virus that infects and replicates within a bacterium?

Motility

Haziness in a SIM medium is indicative of what?

Contractile vacuole

What structure helps amoeba expel excess water out of the cell?