Fermentation test: carbohydrate metabolism
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10 Terms
What is biochemical testing for?
detect the presence of enzymes in bacteria and yeast
What does the fermentation test contain
media has protein, a single carbohydrate, pH indicator dye, and inverted Durham tube
What is the Durham tube used for?
This is used for gas
What does the bacteria use the protein or carbohydrate as a
energy and carbon source
What does the presence of a bubble in the Durham tube indicate?
Gas formation
Can you make gas without changing the pH?
NO
Can some fermenters also make acid AND gas?
Yes
If the tube is the same color after inoculating the bacteria
No fermentation has taken place (maybe that means there’s O2)?
If the tube is changing color
bacteria is fermentation and tube is becoming more acidic
more acidic means
tube color will becoming lighter like red to yellow
