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6 Classes of Enzymes
Oxidoreductases, Transferases, Hydrolases, Lyases, Isomerases, Ligases
Oxidoreductase Purpose
Oxidation-Reduction reaction
Transferases Purpose
Transfer of functional groups
Hydrolases Purpose
Hydrolysis reactions
Lyases purpose
group elimination to form double bonds
Isomerases Purpose
Isomerization reactions
Ligases Purpose
Bond formation coupled with ATP Hydrolysis
How is a Lineweaver-Burk plot effected with a competitive inhibitor
Km increases, Vmax is unchanged
How is a Lineweaver-Burk plot effected with an uncompetitive inhibitor
Km decreases, Vmax decreases
How is a Lineweaver-Burk plot effected with a noncompetitive inhibitor
km is unchanged, Vmax is decreased
What type of inhibitor is this?
noncompetitive
What type of inhibitor is this?
A competitive inhibitor
What type of inhibitor is this?
uncompetitive
What is a competitive inhibitor
A molecule that binds to an enzyme's active site, preventing the substrate from binding
What is a noncompetitive inhibitor
A molecule that binds to an enzyme at a site other than the active site, altering enzyme function and preventing substrate binding.
What is an uncompetitive inhibitor
A molecule that binds only to the enzyme-substrate complex, preventing conversion to the product.