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Fixation for histochemistry temp
0-4 C
Cryostat temp
-18 to -20 C
Melting point of wax in 20-24C
54-58 C (56 C ave)
Wax bath thermostat and automated impregnation temp
3C above melting point of wax
Manual impregnation temp
2-5 C above melting point of wax (55-60)
Flotation water bath temp
6-10 C below melting point of wax (45-50C)