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Extra CH domain
What part of IgE do mast cells bind to?
3
Papain can split the Ig molecule into ___ different fragments of about equal size.
Shingrex
Zostavax
What are 2 vaccination options for Herpes Zoster infections?
Zostavax
What herpes zoster vaccine contains a live, weakened form of the chickenpox (varicella) virus?
Shingrix
What herpes zoster vaccine is NOT a whole weakened form of the virus but instead, contains a surface-sitting protein and 2 adjuvants?
Shingrix
Which herpes zoster vaccine has a higher protective effect after 4 years?
72
Shingles symptoms can be eradicated if treated within _____ hours.
shingles
What infection is shown in the image?
50 & older
At what age can the shingrix vaccine be given?
2 doses
How many doses make up the shingrix vaccine?
Shingrix
Which shingles vaccine is preferred by doctors--Shingrix or Zostavax?
2
If you have already had Zostavas, you should receive ____ doses of Shingrix.
True
True/False: You will not need Zostavax or Shingrix if you have had the chicken pox vaccine.
herpes zoster ophthalmicus
What condition is shown in the image?
Fever
Myalgia
Chills
Yes
What are the 3 main side effects of Shingrix? Is this worse than the Zostavax side effects?
NSAIDs
What medication can be taken to alleviate side effects associated with the Shingrix vaccine?
Fc portion
What portion of the antibody determines the biological activity of the antibody?
soluble; globulins; adaptive
Antibodies are (insoluble/soluble) proteins made of amino acids that circulate freely and contribute to immunity. They belong to a class of proteins called __________ and are important to the (innate/adaptive) immune response.
True
True/False: Antibodies can be either membrane bound or secreted.
plasma cells
What type of cells secrete antibodies?
Fragment Antigen Binding
What does the “Fab” portion of the antibody mean?
Fab portion
What portion of the antibody is specific to a particular antigen?
Fragment Crystallizable
What does “Fc” portion mean?
constant; hypervariable
The Fc portion of an antibody is also known as the _____ region. The Fab portion of an antibody is also known as the ___________ region.
Neutralization of toxins (i.e. animal bite)
Immobilization of microorganisms
Precipitation followed by phagocytosis
Activation of complement
Placental cross (i.e fetal protection)
What are the 5 main functions of the Fc portion of an antibody?
Fab portion
Fc portion
Light chain
Disulfide chain
Heavy chain
Identify regions 1-5 in the image of the antibody structure.
Serum electrophoresis
Bence Jones proteins
Papain
What are the 3 ways in which the structure of antibodies were figured out?
serum electrophoresis
What form of solving antibody structure used electric field to separate the different parts of the antibody?
urine
Homogenous proteins produced by patients with multiple myeloma during Bence Jones proteins testing
Bence Jones proteins
What test is useful for the determination of antibody light chain structures?
Papain
Proteolytic enzyme discovered to split Ig molecule at the hinge region
Fab portion
Fab portion
Fc portion
What are the 3 fragments produced from papain enzyme?
Fc
During the use of papain, the _____ portion cannot bind the antigen and is responsible for biological activity post-antigen binding.
Fab portion
Fc portion
Interchain disulfide bonds
Intrachain disulfide bonds
Light chain hypervariable region
Light chain
Heavy chain
Heavy chain hypervariable region
hinge region
Complement binding region
Identify the following structures of the antibody.
pepsin
The use of ____ enzyme resulted in 2 Fab connected structure and the dissolving of the Fc portion into smaller fragments.
pepsin
What enzyme is indicated by number 1 in the image and results in the fragments presented on the right side?
mercaptoethanol
In figuring out antibody structure, what was used as a disulfide bond breaker that splits Ig into 4 chains?
2 identical light chains & 2 identical heavy chains
What 4 chains does Ig split into when using mercaptoethanol?
disulfide bond
What type of bond holds together the light chains and heavy chains?
hinge region
At what part of the antibody does papain split the immunoglobulin?
Kappa
Gamma
What are the 2 classes of light chains that are present in antibodies of most species?
Protein sequence
Carbohydrate content
Size
Constant regions of heavy chains
What are the 4 distinguishing features of heavy chains?
IgG
IgA
IgM
IgE
IgD
What are the 5 different isotopes/classes of antibodies?
nature of the heavy chains
What determines the difference between the 4 different isotopes of antibodies?
IgA
IgG
Which 2 antibody types have subclasses?
Interchain disulfide bonds
What type of disulfide bonds occur between heavy and light chains?
Intrachain disulfide bonds
What type of disulfide chains occur within heavy and light chains, causing loops?
IgG
_____ field domains form interchain disulfide bonds.
Fab chain
What type of chain is considered to be a variable chain?
Fc chain
What type of chain is also known as a constant chain?
Between CH1& CH2
Between what chains is the hinge region formed?
heavy
The hinge region is located on (heavy/light) chains.
Cysteine
Proline
What amino acid residues are present at the hinge region?
Ig flexibility
What does the hinge region permit?
Interchain disulfide bond
What type of chain is indicated by the blue circle?
Intrachain disulfide bond
What chain is indicated by the red circle?
Isotypes
-Immunoglobulin variant that allows for different biological responses due to differences in constant regions
-IgA, IgD, IgE, IgG, IgM
Alloptypes
-Immunoglobulin variant that is based on genetic differences of members of the same species
-differences due to different alleles of same constant region gene
Idiotypes
Immunoglobulin variant that has differences due to rearrangement of VH and VL encoding genes within a given isotype
Membrane-bound antigen receptor on B cells
Secreted proteins
In what 2 form do antibodies exist?
Membrane-bound antigen receptors
What form of antibodies recognize antigens to start B cell activation?
Secreted proteins
What form of antibody is present in the blood and mucosal secretions and eliminates microbes and other toxins in humoral immunity?
IgG
Most common Ig in blood, lymph fluid, CSF, peritoneal fluid
15
IgG makes up _____% of total body protein.
2; 2
How many heavy chains does IgG contain? Light chains?
4
How many subclasses does IgG contain?
IgG
Which antibody is the workhorse and is the most versatile Ig?
IgG
What Ig had the longest half life?
IgG
What antibody isotype is the best for passive immunization?
precipitated
Phagocytes can rid antigens easier once __________.
precipitate; agglutinate
Soluble antigens (agglutinate/precipitate) while insoluble antigens (agglutinate/precipitate).
IgG
What antibody passes through the placenta to transfer immunity from mom to fetus?
IgG
What antibody is responsible for hemolytic disease of the newborn?
Fc portion
On what part of the IgG antibody do phagocytes attach to?
opsonization
Preparation of antigen before being “eaten” by macrophages
Fc portion
Natural killer cells interact with what part of the antibody of IgG?
IgG
What immunoglobulin is responsible for activation of complement, neutralization of toxins, immobilization of bacteria, and neutralization of viruses?
anti-toxin
To neutralize toxins, injection of an _______ can be used to block the active sites of the toxin.
passive
The injection of anti-toxin is a form of (passive/active) immunization.
IgG
What immunoglobulin reacts with flagella and cilia causing them to clump and immobilize the bacteria?
IgM
What immunoglobulin has the highest molecular weight of all Ig’s?
5; J; 5; 10
IgM has ____ basic Ig units. The __ chain is the connector. IgM has __ binding sites for the antigen but has _____ Fab segments.
antigen-specific
IgM is an ______________ B cell receptor.
IgM
What immunoglobulin is present on the surface of mature B cells along with IgD?
IgM
What is the first Ig produced following immunization or infections?
IgM
What Ig the first line of humoral defense against bacteria?
IgM
Elevated levels of what immunoglobulin indicates recent infection or exposure to an antigen?
IgM
Which immunoglobulin is the best at activating complement?
membrane attack complex
Activation of complement leads to activation of what cell that can attack bacteria?
IgG
Comparing IgM and IgG, what immunoglobulin is highest in the secondary response against T-dependent antigens?
IgM
Comparing IgM and IgG, what immunoglobulin is highest in the primary response against T-dependent antigens?
IgM; congenital or perinatal infection
What immunoglobulin is synthesized by the fetus at about 5 months? Elevated levels of this Ig indicate what?
IgM
What immunoglobulin is an efficient agglutinator and involves isohemagllutinins?
Isohemagglutinins
Naturally occurring antibodies against the RBC antigens of the ABO blood groups
Type O
What blood type has antibodies to A and B antigens?
Serum
Secretions
What are the 2 structural forms of IgA?
IgA
What antibody has no known biologic function?
dimer
Secratory IgA is mainly in the structural form of a _______.
monomer
The serum form of IgA is in the structure form of a ________.
J chain
What chain joins the 2 four-chained units of IgA?