Lecture 4: Mobile Introns and Inteins
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13 Terms
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mobile genetic elements
types of DNA that can move around in the genome; common examples include transposons ("jumping genes") and plasmids
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Group 1 introns
\-Found in rRNA
\-Self splicing
\-Ribozymes
\-diverse but not common
\-Self splicing
\-Ribozymes
\-diverse but not common
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Homing
lateral or horizontal transfer of an intervening sequence to an intron lacking version of the gene
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homing endonuclease
Recognize and cleave 12-40bp DNA sequence motifs
Recognize genes lacking intron
Recognize genes lacking intron
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Group 1 intron intermediate
LINEAR
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Inteins
Protein segments that are removed from the final protein
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Exteins
The protein segments that are kept and spliced together
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Ectopic transposition
Movement of intron to a new genomic site
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How can ectopic transposition occur?
Reverse splicing into another RNA
Reverse Tc
Recombination of cDNA into genome by recombination / repair enzymes
Reverse Tc
Recombination of cDNA into genome by recombination / repair enzymes
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Group 2 introns
protein-encoding genes in mitochondria and chloroplasts, self-splicing
Similar to spliceosomal
Similar to spliceosomal
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Group 2 introns intermediate
LARIAT
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Intron encoded protein
Functions as RNP complex bound to intrinsic RNA that encoded it
Mediates homing to intron-minus location
Mediates homing to intron-minus location
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protein splicing
removal of part of polypeptide before folding
Note 
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