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1Upper Lateral 3rd of Vaginal Wall:
for Evaluation of HORMONAL ACTIVITY
Ectocervix and Endocervix
Other gynecological specimen
T-zone
where pre-cancerous lesions can be detected
Vaginal Scrape
recommended for patient with HYSTERECTOMY
Vulvar Scrape
recommended for detecting HERPECTIC CARCINOMA
Vulvar Scrape
recommended for detecting HERPECTIC LESIONS
4 Quadrant Vaginal Scrape
recommended for the LOCALIZATION of VAGINAL ADENOSIS
Orange Green-6 (OG 6)
Stains the Cytoplasm of SUPERFICIAL CELLS (Counterstain)
Eosin Azure-50 (EA-50)
Stains the Cytoplasm of PARABASAL CELLS and INTERMEDIATE CELLS. (Counterstain)
SUPERFICIAL CELLS
most mature, Polygonal cells with DARK PYKNOTIC NUCLEI
SUPERFICIAL CELLS
Cells that exhibits “TRUE ACIDOPHILIA”
characteristics of vaginal cell under the influence of ESTROGEN
INTERMEDIATE CELLS
medium cells, Polyhedral with BASOPHILIC and VACUOLATED cytoplasm
NAVICULAR CELLS
found in the latter half of MENSTRUAL CYCLE, MENOPAUSE and during pregnancy
NAVICULAR CELLS
Presence suggest Progesterone-Estrogen effect
NAVICULAR CELLS
BOAT-SHAPED CELLS with a tendency to fold or curl on egdes
PARABASAL CELLS
thick ROUND OVAL CELLS,
PARABASAL CELLS
Nucleus is Large (FRIED EGG app.)
PARABASAL CELLS
*normally found in: ABORTION, AFTER MENOPAUSE, AFTER CHILD BIRTH And 2 weeks of AGE to PUBERTY
PREGNANCY CELLS
Nucleus pushed at periphery with double walled boundary appearance
PREGNANCY CELLS
Round oval BOAT SHAPED CELLS with translucent basophilic cytoplasm due to glycogen accumulation.
ENDOMETRIAL CELLS
Occurring in groups of three or more, Found during first week of menstruation
ENDOMETRIAL CELLS
Similar to parabasal but less basophilic.
Slightly cylindrical w less basophilic cytoplasm
ENDOCERVICAL/GLANDULAR CELLS:
Occurs in large group with HONEYCOMB APPEARACE
DODERLAIN BACILLUS/ LACTOBACILLUS ACIDOPHILUS
normal vaginal flora
DODERLAIN BACILLUS/ LACTOBACILLUS ACIDOPHILUS
Last Trimester of Pregnancy
- Infection
- Estrogen deficiency
- Diabetes Mellitus
CANDIDA ALBICANS
yeast cell that is commonly seen in patients with Diabetes, Taking contraceptives and patient with Immunocompromised states.
TRICHOMONAS VAGINALIS
Stains blue to blue gray
GARDNERELLA VAGINALIS
may appear as cells surrounded by bacilli “CLUE CELLS”
KOILOCYTES:
cells that shows CYTOPATHIC EFFECT of Human Papilloma Virus.
KOILOCYTES:
Cells with atypical nucleus surrounded by perinuclear halo.
KOILOCYTES:
Nucleus may appear like WRINKLED PRUNES.
FERNING
phenomenon in which cervical mucus exhibit a “PALM LEAF” pattern on drying
FERNING
- possible to happen in patients with HIGH ESTROGEN LEVEL
FERNING
- can be used for detection of EARLY PREGNANCY
Barr body
XX chromosome
-10 to -15
Testes
-10 to -15
uterine curettage
-15 to -25
Other specimen
-25 to -25
Breast
-25 to -25
Fat
-25 to -25
Lung
-25 to -25
Skin with fat
20-30 mL
Serous fluids Required volume
5-10 mL
Gastric washing Required volume
50-100 mL
Urine Required volume
Deep cough morning sputum
Sputum Required volume
Prostatic/gynecological material
Required volume Cervico-vaginal and endometrial or prostatic smears
Serous fluid
Collection: Tube, syringe, or clean container
Gastric fluid
Collection: During gastroscopy
Urine
Early morning void urine or catheterized
Sputum
Spontaneous or induced collection
Prostatic/gynecologic material
Spatula, endocervical brush, and cytobrush during midcycle for women
Serous fluid
Refrigeration, wet ice on transport, or addition of equal amount of 10% formalin
Gastric washing
Delivered in ice packs
Urine
Refrigeration
Sputum
95%ethyl alcohol or 10% formalin
Prostatic/gynecologic material
95% Ethyl alcohol
Note 
Flashcard (33)