DNA replication

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What is replication and where does it happen (2+1)

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1

What is replication and where does it happen (2+1)

  • Creating a copy

  • Forming a leading and lagging strand

  • In the nucleus

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2

What direction does DNA polymerase cream rage new DNA strand in

5’-3’, one nucleotide at a time

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3

What is the 5’ end

Phosphate end

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4

What is the 3’ end

Deoxyribose end

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5

How does DNA run

Antiparallel (opposite directions to each other)

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6

What is the enzyme that separates the strands of DNA

DNA helicase

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7

What bonds hold the two strands together

Hydrogen bonds

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8

Which enzyme allows DNA polymerase to bond

DNA primase

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9

Which end does DNA primase attach

3’ end (nearest helicase in lagging strand)

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10

How is the new strand of DNA made in the leading strand (3)

Continuously in a 5’-3’ direction using complementary bases

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11

How is the new strand held to the old strand

Hydrogen bonds

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12

How are lagging strands

In Okazaki fragments

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13

How are Okazaki fragments attached together

DNA ligase seals the gaps in the sugar phosphate backbone with phosphodiester bonds

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