DNA replication

What is replication and where does it happen (2+1)

• Creating a copy

• Forming a leading and lagging strand

• In the nucleus

What direction does DNA polymerase cream rage new DNA strand in

5’-3’, one nucleotide at a time

What is the 5’ end

Phosphate end

What is the 3’ end

Deoxyribose end

How does DNA run

Antiparallel (opposite directions to each other)

What is the enzyme that separates the strands of DNA

DNA helicase

What bonds hold the two strands together

Hydrogen bonds

Which enzyme allows DNA polymerase to bond

DNA primase

Which end does DNA primase attach

3’ end (nearest helicase in lagging strand)

How is the new strand of DNA made in the leading strand (3)

Continuously in a 5’-3’ direction using complementary bases

How is the new strand held to the old strand

Hydrogen bonds

How are lagging strands

In Okazaki fragments

How are Okazaki fragments attached together

DNA ligase seals the gaps in the sugar phosphate backbone with phosphodiester bonds