minimum crieria and ID techniques for colonies

GPC flow chart, min crit, BC results

what is the first step of the GPC flow chart

catalase

what is the next step after a positive catalase test on the GPC flow chart

staph latex

What microbe is GPC, catalase +, staph latex =

SCNG

what is the next step after a negative catalase test on GPC

what is the hemolysis pattern shown

what is the next step after a negative catalase test on a GPC with an alpha/gamma hemolysis pattern

a pyr test

what alpha/gamma hemolysis GPC with a neg catalase test and a positive PYR

enterococcus spp

what alpha/gamma hemolysis GPC with a neg catalase test and a negative PYR

alpha or gamma strep

what is the next step after a GPC has a negative catalase test and beta hemolysis

strep typing

what is done to a GPC after a negative catalase test, beta hemolysis, and a negative strep typing test

PYR

GPC, catalase =, beta hemolysis, neg Strep typing, neg PYR

beta strep that is not A, B, or C serotypes

GPC, catalase =, beta hemolysis, neg Strep typing, pos PYR

enterococcus spp

What does a PBP2a differentiate

S. aureus and MRSA

what test is done after a GPC has a pos staph latex test

a pbp2a is performed

when is a MRSA ID notified to the floor

when it is found in the blood or CSF

when are min crits not allowed to be used

isolates that are from blood, CSF, brain, or cardiac specimens UNLESS S. aureus

what should be utilized in EPIC when using min crit

the corresponding workcards

Is Pseudomonas aeruginosa a presumptive or definitive ID

Presumptive

Is E. coli a presumptive or definitive ID

presumptive

Is Proteus mirabilis a presumptive or definitive ID

presumptive

Is Proteus vulgaris a presumptive or definitive ID

presumptive

Is Candida albicans a presumptive or definitive ID

presumptive

Is S. aureus a presumptive or definitive ID

definitive!

Is Streptococcus pyogenes a presumptive or definitive ID

definitive

Is Streptococcus agalactiae a presumptive or definitive ID

definitive

Is Streptococcus sero group C a presumptive or definitive ID

definitive

are Enterococcus spp a presumptive or definitive ID

presumptive

Is coagulase neg Staphylococcus a presumptive or definitive ID

definitive

are diptheroids a presumptive or definitive ID

presumptive

Is strep spp alpha hemolytic not enterococcus a presumptive or definitive ID

presumptive

Is lactobacillus a presumptive or definitive ID

presumptive

what is the min crit required for Pseudomonas aeruginosa

Flat, spreading or metalic on Bap, neg lactose fermentation, indole neg, oxidase pos

how many characteristics need to match to make a presumptive ID

all of the them

what is the min crit required for E. coli

flat/non-mucoid colonies w/ bile precipitate on MAC, pos lactose ferment,indole pos, oxidase neg

what is the min crit required for P. mirabilis

Swarming, neg lactose ferment, indole neg, oxidase neg

what is the min crit required for Proteus vulgaris

swarming, neg lactose ferment, indole pos, oxidase neg

what is the min crit required for Candida albicans

Gram stain/wet prep pos for yeast, white colony color, feet/ extensions from colony

when do yeast need to be ID via maldi or Vitek 2

if sample is from CSF, blood, brain, or cardiac sites

what is the min crit required for S. aureus

GPC, beta hemolysis, yellow/white colonies, catalase pos, staph latex pos

What organism can be ID using min crit from all body sites

S. aureus

what is the min crit required for Strep pyogenes

GPC, beta hemolysis, light gray colonies, neg catalase, strep A typing

what is the min crit required for Strep agalactiae

GPC, beta/gamma hemolysis, clear/colorless colonies, catalase neg, strep B typing

what is the min crit required for Strep C group

GPC, beta hemolysis, catalase neg, strep C typing

what is the min crit required for Enterococcus spp

GPC, alpha/gamma hemolysis, gray colonies, catalase neg, PYR pos

what is the min crit required for coagulase neg staph

GPC, beta/gamma hemolysis, white colonies, catalase pos, neg staph latex

what is the min crit required for diptheroids

GPB (chinese letter formation) white, tiny, dry colonies

what is the min crit required for strep spp alpha hemolytic non enterococcus

GPC, alpha/gamma hemolysis, catalase neg, pyr neg

what is the min crit required for lactobacillus

GPB, alpha hemolysis, catalase neg, usually long and thin under gram stain

E. coli indole

pos

E.coli oxidase

neg

E. coli lactose fermentation

pos

E.coli colony description

flat/non mucoid

P mirabilis indole

neg

P mirabilis oxidase

neg

P. mirabilis lactose fermentation

neg

p. miralibis colony morph

swarming

Pseudomonas aeruginosa indole

neg

Pseudomonas aeruginosa oxidase

pos

p aeruginosa lactose ferment

neg

p aeruginosa colony morph

flat spreading, metalic

p vulgaris indole

pos

p vulgaris oxidase

neg

p vulgaris lactose ferment

neg

p vulgaris colony morph

swarming

S. aureus gram stain

GPC

S. aureus hemolysis

beta

S. aureus catalse

pos

S. aureus staph latex

pos

s. aureus colony morph

yellow/white

Strep A gram stain

GPC

strep A hemolysis

beta

strep A catalase

neg

Strep agalactiae gram stain

GPC

strep B catalase

neg

Enterococcus gram stain

GPC

enterococcus hemolysis

alpha/gamma

enterococcus catalase

neg

enterococcus pyr

pos

CNSC gram stain

GPC

CNSC catalase

pos

CNSC staph latex

neg

diptheroid gram stain

GPB in pallisade

Strep, alpha hemolytic, not enterococcus catalse and pyr

both neg

what needs to be done after a presumptive ID is made on a microbe

MALDI