Final Reference Ranges

What is the reference range for the myeloid:erythroid ratio?

1.5:1 - 5:1

What cells are included in the erythroids?

Nucleated RBCs

What cell population is the M:E ratio used for?

BM

How do you calculate M:E ratio?

Divide both sides by erythroid cells

An RPI < 2 signals what kind of defects?

1. Proliferation Defects

2. Maturation defects

What is the BM in proliferation defects?

Erythroid hypoplasia

What are two disorders stemming from Proliferation defects?

1. Aplastic anemia

2. Neoplastic disorders

What is the BM and pathology for maturational defects?

1. Erythroid hyperplasia

2. Ineffective EPO

What are two disorders associated with maturational defects?

1. Megaloblastic Anemia

2. Iron Deficiency Anemia

What is a feature of the BM in maturation defects?

Decreased retic count

What kind of defect does an RPI > 2 signify?

Survival defect

What are two features of the BM in a survival defect?

1. BM hyperplastic

2. Increased retic count

What are two disorders associated with survival defects?

1. Hemorrhage

2. Hemolytic Anemia

What does an increased RDW mean?

Great variation in cell size

What does a normal RDW mean?

Uniform size

What does a decreased RDW mean?

Not significant

What is the RDW reference range?

12-15%

MCV reference range?

80-100 fL

MCH reference range

26-34 pg

MCHC reference range

31-37 g/dL

How do you calculate MCV?

(HCT x 10)/(RBC count)

How do you calculate MCH?

(HGB x 10)/(RBC count)

How do you calculate MCHC?

(HGB x 100)/(%HCT)

How do you calculate HCT?

(RBC x MCV)/(10)

What is the HCT reference range male and female?

1. Male: 41-53%

2. Female: 36-46%

RBC count for male and female

1. Male: 4.5-5.9 × 10^12/L

2. Female: 4.0-5.2 × 10^12/L

HGB reference range male and female

1. Male: 13.5-17.5 g/dL

2. Female: 12.0-16.0 g/dL

B-cell CD markers

1. CD 19

2. CD 20

3. CD 21

4. CD 23

5. K and gamma light chain

Myeloid CD markers

1. CD 11/CD 11c

2. CD 13

3. CD 15, CD 64 (monocyte)

4. CD 33

5. CD 117

CD markers T-cells

1. CD 1a

2. CD 2

3. CD 3

4. CD 4

5. CD 5

CD Megakaryocyte

1. CD 41

2. CD 42

3. CD 61

CD marker for early lymphs?

TdT

CD marker for ALL antigen?

CD 10

CD marker for stem/progenitor cell

CD 34

CD marker for pan leukocyte

CD 45

CD marker for monocytes

CD 14

CD marker for TfR RBC’s?

CD 71

CD marker for plasma cells

CD 38

What does glycophorin A correspond to?

RBCs

What karyotype and gene correspond to CML?

1. Karyotype: t(9;22)

2. Gene: BCR/ABL1

What karyotype and gene corresponds to Polycythemia Vera?

1. Karyotype: 9p24

2. Gene: JAK2(V617F)

What karyotype and gene corresponds to Burkitt’s Lymphoma?

1. Karyotype: t(8;14)

2. Gene: MYC/IGH

What is transferrin and what is the reference range?

1. Iron-transporting protein

2. 200-370 mg/dL

What is Serum Fe and what is the reference range and measurement method?

1. Amount of iron available for EPO

2. 35-165 ug/dL

3. Calorimetry

What is TIBC?

1. Total Iron-Binding Capacity

2. Amount of iron that can functionally bind to Transferrin

How do you calculate transferrin saturation, and what is the reference range?

1. TS = (Serum Fe/TIBC) x 100

2. 25-35%

What is TfR, what does it reflect, how is it related to body iron, how is it detected?

1. Transferrin Receptor

2. Reflects cellular TfR

3. Inversely proportional to body iron

4. Immunoassay

What is TfR used for?

Differentiates IDA from ACD