MLB4710 Chapter 14 Staphylcocci and Micrococci

Part 1 - Staph Agars and Biochemical Reactions

TSA with 5% BAP is (non-selective or selective)

Non-selective

MSA is _____________ and _____________ (type of medium)

Selective; differential

Contents of MSA

7.5-10% NaCl = selective

Mannitol

Phenol red indicator

______________ is positive for mannitol fermentation (yellow)

S. aureus

______________ is negative for mannitol fermentation (no color change, red)

S. epidermidis

ChromAgar can be used to selectively grow _______

MRSA

Staph. species are catalase ________

Positive

S. aureus produces __________ (type of hemolysis)

B-hemolysis

Staph. is an __________ or ___________ (O2 requirements)

Aerobe; facultative anaerobe

Hemolysis pattern — complete lysing of RBCs

B-hemolysis

Hemolysis pattern — partial lysing of RBCs (green-ish)

a-hemolysis

Hemolysis pattern — no lysing of RBCs

Gamma hemolysis