how to generate DNA profiles

what does RFU stand for

relative fluorescence units

what does the vertical axis show in capillary electrophoresis

the RFU

what does the RFU show

the intensity of light picked up by camera in detector window at different points of time

what is the horizontal axis measured in

mins or seconds

what are the first peaks usually representing in an electropherogram

primers left over from PCR

what does an electrophoregram of an EPG show us

info captured by a computer and peaks pulled apart to show component colours

what are the 4 colours seen in an EPG

green, blue, yellow and red w

what can yellow also be shown as for contast

black

how many loci can be in an EGS

9

what are the 9 loci

• D3

• VWA

• FGA

• D8

• D21

• D18

• D5

• D1

• D7

what are the peaks that we see in the EPG

individual alleles

What is na example of a non polymorphic locus

amiolgenin

where can amiolgenin be found

on the far left side

what does it mean if there are 2 peaks at amiolgenin

X,Y chromosome, male profile

what are the red peaks for

internal size standards

what does homozygous mean

having 2 identical alleles at a locus

what does heterozygous mean

having 2 different alleles at a locus

what does 3 or more peaks at a locus mean

mixed sample

what are humans known as

diploid

what does diploid mean

having 2 copies of genetic data

what do peaks on the left side of an EPG show us

small fragments of DNA

what do peaks on the right side show us

large fragments of DNA

where will the larger alleles always appear

on the right

why will the larger alleles always be on the right

they take longest to make it through the capillary

what does the height of the peak show

amount of DNA that gave rise to that peak during PCR amplification

what are some alternative hypothesis to finding a match (3)

• error in handling after collection

• contamination

• deliberate mistake