Studied by 0 people
Looks like no one added any tags here yet for you.
Gel Electrophoresis
A technique used to separate charged molecules, such as proteins, in an electric field.
Polyacrylamide Gels
A matrix used in gel electrophoresis to sieve protein molecules based on size and charge.
Native PAGE
A type of polyacrylamide gel electrophoresis that maintains the proteins' natural state without denaturing them.
Charge to Mass Ratio
A constant ratio required for proportional movement of proteins in gel electrophoresis.
SDS-PAGE
A method that denatures proteins and provides a constant charge-to-mass ratio for separation based on size.
Sodium Dodecyl Sulfate (SDS)
An amphipathic molecule used to denature proteins and impart a negative charge during SDS-PAGE.
Mercaptoethanol
A reducing agent used to break disulfide bonds in proteins during SDS-PAGE.
Isoelectric Point (pI)
The pH at which a protein has a net charge of zero and does not migrate in an electric field.
pH Gradient
A stable gradient established in isoelectric focusing that influences protein migration based on their pI.
2-Dimensional PAGE
A technique combining isoelectric focusing and SDS-PAGE for high-resolution separation of proteins.
Proteomics
The study of the complete set of proteins (proteome) in an organism.
Genome
The complete DNA sequence of an organism, containing all its genes.
Transcriptome
The complete set of RNA molecules transcribed from a genome.
Metabolome
The complete set of small molecules found in a cell.
Protein Purification
The process of isolating a pure protein from a mixture for detailed study.
Ion Exchange Chromatography
A technique that separates proteins based on their charge by binding positively charged proteins to beads.
Size Exclusion Chromatography
A method that separates proteins based on size, allowing larger proteins to elute faster than smaller ones.
Affinity Chromatography
A technique that uses specific binding interactions to isolate proteins with a tag.
His-tag
A sequence of six histidine residues used to facilitate the purification of proteins via affinity chromatography.
Overexpression
The process of increasing the production of a specific protein, often through cloning, to facilitate purification.
Note 
Flashcard (80)