B1.2.5 - Denaturation of Proteins

Define denaturation

The process in which proteins lose their native conformation and function due to environmental factors.

Why does denaturation occur in proteins?

Because the dimension of proteins are stabilized by bonds or interactions (e. hydrogen bonds) b/w the R-groups, but they are relatively weak

→ they can easily be disrupted or broken

Why is denaturation often permanent?

a denatured protein forms new bonds to stabilize the new structure

How does heat cause denaturation?

Vibrations in the molecule → breaks intermolecular bonds/ interaction

how do extreme pHs cause protein denaturation?

It changes the attraction of the pos.& neg attractions on the R-groups + introduces more or less hydrogen → breaks and/or forms new bonds → changes the conformation

Why do denatured proteins often turn soluble → insoluble?

because the hydrophobic parts that usually face in side are not turned outwards

What is a common example of protein denaturation in food?

albumin denaturation in egg whites

Explain how the denaturation of albumin occurs when eggs are heated

added heat → vibrations → breaks hydrogen bonds & cross linkages (-SH bridges) → new bonds (S-S, disulfide bridges) are formed + egg white is insoluble & opaque