biol 300 final

DNA Polymerase

Better Proofreading capacity

RNA Polymerase

Binds to promoter sequence

DNA Polymerase

Only Active during S phase of mitosis

DNA Polymerase

Faster

Both DNA and RNA polymerase

Forms phosphodiester bonds

DNA Polymerase

requires RNA primer

RNA polymerase

adds ribonucleotides to 3’ end of new polymer

Both DNA and RNA polymerase

works in conjunction with other proteins

Eukaryotes

transcription in nucleus

Prokaryotes

RNA polymerase holoenzyme with sigma unit

Eukaryotes

Enhancers and activators form protein bridges

Eukaryotes

RNA processing begins during transcription

Eukaryotes

Transcription factors: TFIID, TFIIA, TFIIB, TFIIF

Prokaryotes

Transcription coupled with translation

Eukaryotes

polyadenylation signal sequence in 3’ UTR

Prokaryotes

Polycistronic mRNA