DNA Detection

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12 Terms

1
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What is an example of next generation sequencing

sequencing by synthesis

2
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What is the first step of sequencing by synthesis

fragmentation

3
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what is the name of the protein that cleaves the DNA during fragmentation during SBS

transposome

4
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What is added to the ends of the DNA after fragmentation

adapters

5
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What step comes after adapter addition in SBS

bridge amplification

6
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which strand is washed away during the bridge amplification step

parent strand

7
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what are the clusters of the same DNA fragments called in SBS

monoclonal clusters

8
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what is used to label dNTPs in SBS

fluorophore

9
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Apart from the fluorescent tag on dNTPs in SBS what is added

reversible terminator group

10
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how is reversible terminator group removed

cleavage buffer

11
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what is the most common type of restriction endonuclease

type 2

12
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what kind of sequences do type 2 restriction endonucleases bind to

palindromic