Tools for Analyzing Gene Expression Part 2

These flashcards cover various tools and techniques for analyzing gene expression, including methods for studying interactions and protein structures.

ELISA

Enzyme-Linked Immunosorbent Assay, a quantitative method similar to Western Blot that requires a specific antibody for the protein.

EMSA

Electrophoretic Mobility Shift Assay, a technique that detects protein-nucleic acid interactions based on size differences during electrophoresis.

Co-Immunoprecipitation (Co-IP)

A method that uses an antibody to capture a protein from a sample, allowing for the detection of interacting proteins.

X-ray Crystallography

A technique used to determine the 3-dimensional structure of proteins by analyzing the diffraction patterns of X-rays shot through crystallized protein.

Protein Structure

The arrangement of amino acids in a protein, which can be predicted to some extent based on the behaviors of the amino acids.

Supershifts

An added step in the EMSA that involves using an antibody to confirm the presence of a bound protein by shifting the band higher in the gel.

In vitro mutagenesis

A method to introduce mutations into a DNA sequence in a controlled environment.

RNA interference (RNAi)

A biological process in which RNA molecules inhibit gene expression or translation by neutralizing targeted mRNA molecules.

Western Blot

A laboratory method used to detect specific proteins in a sample using gel electrophoresis followed by transfer to a membrane and probing with antibodies.

Chromatin immunoprecipitation (ChIP) assay

A technique used to determine the location of DNA-binding proteins on the genome.

Atomic force microscopy (AFM)

A type of scanning probe microscopy that provides high-resolution imaging of surfaces at the nanoscale.

Northern blot

A technique used to study gene expression by detection of RNA in a sample.