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Pope Innocent VII;1492

recipient of first blood transfusion in history, but he & the donors died

Karl Landsteiner;1901

discovered ABO blood groups and explained transfusion reaction

Alfred von Decastello and Adriano Sturli;1902

discovered the fourth ABO blood group, AB

Edward Lindemann;1913

used vein-to-vein transfusion of blood by using multiple syringes

Albert Hustin;1914

used sodium citrate as Ac for indirect blood transfusion

Francis Rous and J.R. Turner;1916

introduced citrate-dextrose solution for preservation of blood

Karl Landsteiner and Alex Wiener; 1940

Rh blood group was discovered

Dr. Charles Drew;1941

appointed director of first American Red Cross bloodbank;established the national volunteer blood program

Robin Coombs, Arthur Mourant and Rob Race;1945

describe the use of Anti-Human Globulin

George Kohler and Cesar Milstein;1975

discovered monoclonal antibodies thru Hybridoma technology

1-6 °c

storage temp. of liquid whole blood / RBC units

1-10 °c

shipping/transport temp. of liquid whole blood / RBC units

20-24 °c

platelet and granulocyte concentration

35 days of shelf-life

CPDA-1

21 days of shelf-life

ACD-A, CPD, CP2D

ACD-A

Acid-citrate-dextrose Formula A

CPDA-1

Citrate-phosphate-dextrose-adenine

has lower pH; loss of 2,3 DPG @ 1st week

ACD-A

Phosphate preserves 2,3-DPG

CPD

Adenine maintains ATP levels

CPDA-1

100% more glucose than CPD

CP2D

42 days

Additive solution shelf life

24 hours

Rejuvenation solution shelf life

10 years

Red cell Freezing shelf life

Added to RBCs within the first 72 hours of storage

Additive solution

Can be used up to 3 days

after expiration of red cells

Rejuvenation solution

Includes addition of a cryoprotective agent to RBCs that are < 6 days old

Red cell Freezing

Red cell Freezing content

Most common cryoprotective agent is Glycerol (40% w/v or 20% w/v)

Rejuvenation solution content

PAPI

Phosphate

Adenine

Pyruvate

Inosine

Additive solution content

MAGS

Mannitol

Adenine

Glucose

Saline