Cytochemical Staining

Myeloperoxidase (MPO)

Neutrophils

Monocytes (+ or -)

Eosinophils (except cats)

Sudan black B (SBB)

Neutrophils

Monocytes (+ or -)

Eosinophils (except cats)

Chloroacetate esterase (CAE)

Neutrophils

Eosinophils (+ or -)

Basophils

Megakaryocytes (+ or -)

NOT monocytes

MCT

ALP

Lymphocyte (+ or -)

Immature monocytes

Eosinophils

Basophils (+ or -)

Osteosarcoma

Alpha-naphthyl butyrate esterase (aNBE)

Monocytes (diffuse)

Lymphocytes (focal or -)

Megakaryocytes (+ or -)

Histiocytoma

T-lymphoblastic lymphoma/leukemia

Morin

Aluminum (e.g. Merial rabies vax adjuvant)

Fontana-Masson

Melanoma

Distinguishing melanin from hemosiderin

Phaeohyphomycosis

Gomori methenamine silver (GMS)

Fungal organisms

Actinomyces & Nocardia

Oomycetes (Pythium, Lagenidium)

Periodic acid-schiff (PAS)

Fungal organisms

Coccidioides cell wall purple, endospores red

Immunoglobulin inclusions in splenic MZL (rare)

Mucicarmine

Cryptococcus cell walls

Acid-fast

Mycobacteria

Nocardia (variable)

Alcian blue

Myxoid variant liposarcoma

Papanicolau

Highlighting nuclear features (e.g. highly granulated MCT)

Omega-exonuclease

MCT

Toluidine blue

MCT

Giemsa

MCT

Von Kossa

Calcinosis cutis/circumscripta

Alizarin red S

Calcinosis cutis/circumscripta

Gamna-Gandy bodies

Alpha-naphthyl acetate esterase (aNAE)

T-lymphoblastic lymphoma/leukemia (focal/dots)

Acid phosphatase

T-lymphoblastic lymphoma/leukemia (focal)

Large peripheral T-cell lymphoma (focal)

Prussian blue

Hemosiderophages

Gamna-Gandy bodies