Protein Purification and Isolation

These flashcards cover key vocabulary related to the concepts of protein purification and isolation as taught in the BISC-220 Cell Biology and Physiology course.

Protein Isolation

The process of extracting a specific protein from a cell or tissue sample for study.

Affinity Chromatography

A technique used to purify proteins based on specific interactions between the protein and a ligand attached to a column.

Enzyme-linked Immunosorbent Assay (ELISA)

A popular assay used to detect and quantify proteins, requiring specific antibodies to identify the protein of interest.

Western Blotting

A method that combines protein isolation steps and allows for the detection of specific proteins based on size.

Centrifugation

A process used to separate components of a sample based on density, commonly used to isolate proteins from digested cells.

Ligand

A molecule that binds specifically to another molecule, often used in affinity chromatography to capture target proteins.

Gel Electrophoresis

A technique used to separate proteins or nucleic acids based on size when analyzing samples.

Immunodetection

The process of detecting a specific protein in a sample, commonly used in conjunction with techniques like Western blotting.