Inhibitors Ox Phos and selected values for gibbs free

What does Rotenone inhibit 

Transfer of electrons from Complex I to coenzyme Q

What does amytal inhibit

Transfer of electrons from Complex I to coenzyme Q

What does Antimycin C inhibit

Transfer of electrons from Complex III to cytochrome c

What does Carbon monoxide (CO) Inhibit 

Transfer of electrons from Complex IV to oxygen

What does Cyanide (CN⁻) inhibit

Transfer of electrons through Complex IV to oxygen

what does Atractyloside Inhibit

Inhibits the adenine nucleotide translocase (ANT)

What does Oligomycin Inhibit

Inhibits proton flow through the F₀ component of ATP synthase

what does Dinitrophenol (DNP) do

An uncoupler; facilitates proton transfer across the inner mitochondrial membrane

What does Valinomycin do? 

A potassium ionophore; facilitates potassium ion transfer across the inner mitochondrial membrane

Malonate

Inhibits of Complex II by binding to the active site of the enzyme without reacting.

How does valinomycin inhibit Oxidative Phosphorylation

It disrupts membrane potential, which uncouples oxidative phosphorylation indirectly.

How does Dinitrophenol (DNP) inhibit?

lets protons leak back into matrix, causing the ETC to speed up but ATP synthase can’t use gradient, and then energy is lost as heat.

ΔG°’ for 1,3-Bisphosphoglycerate (1,3-BPG)

-11.8

ΔG°’ Phosphoenolpyruvate (PEP)

−14.8

ΔG°’ Creatine phosphate (Phosphocreatine)

−10.3

ΔG°’ Acetyl-CoA

−7.5