Microbiology Lecture Notes

Flashcards to help review lecture notes.

Before lab you should be __.

Reading the labs and doing any Web-based or hard-copy pre-lab activities.

At the beginning and end of each lab, you should __.

Clean your work area with the spray disinfectant provided.

Lab coats should be worn at all times to protect you against __.

Not only microbes but also chemicals and your Bunsen burner flame.

If you injure yourself in any way during the laboratory, __.

Notify your professor or the lab staff immediately.

Safety doesn't have to be complicated: __

Be observant and use common sense!

You will be using the same microscope throughout this course so you should __.

Handle the microscope with care and clean it thoroughly at the end of each class.

At the end of the lab period, students should __.

Clean all optical components (objective, ocular, condenser, and illuminator) with lens paper and lens cleaner.

When labeling plates, __.

Use a permanent marker to write on the bottom of the plate, not the lid.

__ to prevent water from the lid dropping onto your cultures.

Always place plates in the incubator lid side down.

It is essential that you understand the basics of aseptic technique to __.

Prevent contamination of the sample, others, the environment, or yourself.

When finished with your transfer, __.

Flame your loop again to incinerate any bacteria remaining on the tool.

Before replacing the cap, __.

Make sure to flame the tube again.

Remember to shut the hot plate off at the end of lab, to avoid __

The hot plate might burn you or the equipment

What is false motility?

A process in which cells are repelled by another

__ are attracted, and bind, to negatively charged moieties.

Basic stains have a positively charged chromophore.

What are the cell morphologies?

The three most common shapes of bacteria are the bacillus, coccus, and spirillum.

__ are Akinetes

Reproductive structures; They are large, usually about two to three times larger than the average cell in a filamentous strand.

__ are Heterocysts

Thick-walled cells responsible for nitrogen fixation.

__ Trichomes

Strands of filamentous cyanobacteria contained within a sheath.

The four major groupings of protozoa are organized based on the way __

They use flagella, those that use cilia, those that use amoeboid motion, and parasites lack motility

What role do Fungi play?

Are both friend and foe in one way or another.

What does Fungi Kingdom do?

Either unicellular or multicellular forms.

Smears need to be spread adequately.

It may cause uneven staining and will not heat-fix properly.

S. salivarius streptococcus

Is able to cause the spread of some diseases, such as bacterial meningitis, in postpartum women.

What is Phycology

The study of algae

What is direct staining?

Is known as a simple microscope. When simple stains get done by using basic stains, all of the cells on the slide are stained the same color.

What is bacteria infections can cause?

It may cause the death patient in some file instances.

What is the process done in the microbiology lab?

Includes a wide array of viruses, bacteria, infections and can damage the lenses and illuminators.

What do pathogenic bacteria use flagella for?

The ability to move inside the host

Proper application of Aseptic technique

is important not only in the laboratory but also in the hospital or clinical environment

Groups of microbial phototrophs.

Algae, Cyanobacteria

Adjust the oculars until

a single circle of light is seen.

The hospital in question instituted new infection-control procedures during spinal procedures, including the use of a mask and strict adherence to aseptic technique to avoid

S. salivarius

A chemotherapeutic course of amphotericin B and 5-fluorocytosine was implemented to get what value of a white blood cell after a blood cultures

1,000 per cubic milliliter