mod 3 microscopy

Use of glass lenses to enlarge objects dates back to …

the 1600s

The most important resource for studying biology at the microscopic level

Microscope

The first microscopes visualized …

Protozoans (called animalcule s)

Supplement to microscopy

Staining and immunohistochemistry for clearer identification of cells, molecules and substructures

Ancient people thought diseases were linked with what?

Character, karma, bad deeds/cursed

Hippocrates

• Died in 390 BC

• Questioned link between environment and disease

Ancient methods to curb disease:

Quarantine, roman freshwater acqueducts, hygiene, building planning

1674

First microscopes

Anthonie van Leeuwenhoek

Examined drop off water and saw algae

Microscopy =

Using light or electronics to magnify objects

The units must be __ than the object being measured

smaller-scale

Micrometer used for …

measuring cells

1/1 000 000

Nanometer used to …

measure size of cellular organelles and viruses

1/1 000 000 000

Magnification =

Increase in size of an object

Resolution =

Ability to distinguish two points that are close to each other

Describe improvement of magnification

Contrast =

Difference of light absorbance between two objects or the object and its background

Describe improvement of magnification