DNA Transcription

How many subunits does prokaryotic RNA Pol have

5

what are the subunits of prokaryotic RNA Pol

alpha1, alpha2, beta, beta’, omega

what are the alpha subunits for in prokaryotic RNA Pol

scaffolding

what is the beta subunit of prokaryotic RNA Pol for

catalytic site

what is the beta’ subunit for in prokaryotic RNA Pol

DNA binding

what is the omega subunit of prokaryotic RNA Pol for

stability

what is the initiation factor in prokaryotes called

sigma factor

what subunit of prokaryotic RNA Pol binds to UP element

c terminal domain

what element of sigma70 binds to the -10 region

sigma 2

what element of sigma70 binds to the -35 region

sigma 4

what are the different regulatory elements in eukaryotes

BRE, TATA box, INR, MTE, DPE, DCE

what components make up the TFIID

TBP, TAFs

What does TBP stand for

TATA binding protein

Which transcription factor stabilizes TFIID and allows it to bind to TATA box

TFIIA

What region does TFIID bind to

TATA box

What three things does TFIIB bind to

BRE, TBP, RNA Pol

interaction between which two transcription factors forms clamp like structure

TFIIE and TFIIF

what transcription factor is recruited to act as a helicase

TFIIH

what is the name of the complex which contains 20 enzymes which mediates interactions between activators, repressors, RNA Pol and DNA

Mediator complex

What is the name of the RNA synthesis when only 2-9 bp RNAs are made and degraded

abortive synthesis

what is the name of the transcription complex when it is able to synthesize more than 9bp sequences

open complex

what metal ion does RNA Pol have that attracts rNTPs to RNA Pol

Mg2+

what is the specific RNA Pol used in eukaryotic transcription

RNA Pol 2 

what are the two types of transcription termination in prokaryotes

intrinsic, Rho-dependent

what bases is the terminator sequence in prokaryotes rich in

GC

what formation is created by the terminator sequence

Hairpin loop

what does hairpin loop cause

destabilization of RNA Pol complex

what is the name of the site Rho binds to during prokaryotic transcription termination

Rut site

what bases is the Rut site rich in

C

what molecule does the Rho hydrolyze in order to translocate along DNA

ATP

what does Rho do once it reaches DNA-RNA hybrid

unwinds it

what are the two types of eukaryotic transcription terminations

allosteric, torpedo

during allosteric termination, what two factors bind to C-terminal domain of RNA Pol 2

CPSF, CSTF

what does CPSF

cleavage and polyadenylation specificity factor

what does CSTF stand for

cleavage stimulation factor

which one of the factors attached to the CTD region of RNA Pol 2 recognizes Poly A signal

CPSF

What enzyme degrades uncapped DNA during torpedo termination

RNase

due to what signal does RNase stop degrading RNA

Poly A