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Microfilaments provide scaffhold
Myosin Based Motility
Microtubules provide roadways
Dyneins and Kinesins
Conventional Myosin
Myosin II (self assemble -bipolar filaments)
Unconventional Myosin
Myosin I, III-XI
Myosin II: Domain Organization
Heavy Chain is a motor (ATPase)
Light chains rigidify “neck”
Myosin I
Crosslink Actin Filaments to the PM
Move vesicles along actin filaments
Actin filament alignments and sliding
Sacromere
Basic unit of skeletal and cardiac muscle
Actin Linked Regulation
Tropomyosin: blocks binding site
Troponin: Activated by high Ca2+
Myosin Linked Regulation
Ca2+/Calmodulin dependent Kinase: MLCK (extend)
