Adhesives

Fishing out

done after sections are floated out on a water bath

10 degree celsius

water bath should have a temperature that is __________ lower than the melting point of the wax

Orientation

Correct positioning of the tissue section/ribbon on the slide

Adhesives

mixtures are spread thinly on a clean, grease-free slide

Adhesives

Application: A small drop is smeared over the slide (using a finger, sponge, or another slide) and excess is removed.

Adhesives

Purpose: Promotes proper adhesion of tissue sections during processing.

Adhesives

● Alternative to drying

● Performed to

prevent washing out of tissue sections during

staining

Adhesives

It is a substance which can be smeared onto the slides so that the sections stick to the slides

Adhesives

Not ne cessary for routine staining

Adhesives

Essential for methods that require exposure of sections to acids and alkalis during staining

clean grease-free

If ______________ slides are used and sections are adequately dried, the sections will not float off during staining and adhesive will not be necessary.

Mayer's Egg Albumin

● Most commonly used

Mayer's Egg Albumin

○ Egg white 50 ml

○ Glycerin 50 ml

○ Thymol

Thymol

to prevent the growth of molds

Mayer's Egg Albumin

usually smeared into the clean glass slide before sections are oriented

Mayer's Egg Albumin

Advantage - very easy to make, convenient, and inexpensive.

Mayer's Egg Albumin

Disadvantage - it retains some of the stain and gives a dirty background.

Dried albumin

Dried Albumin + NaCl + Thymol

Dried albumin

Sections are dried and stored in 70% alcohol until it is ready for staining

1% Gelatin

Gelatin + Glycerol + Phenol

1% Gelatin

Added to the water bath during flotation rather than applying it on slides

Gelatin-formaldehyde mixture

● Gelatin + Formaldehyde

Gelatin-formaldehyde mixture

slides are coated and allowed to dry at 37°C for one hour or overnight prior to use

Poly-L-Lysine

Aqueous detergent diluted to 0.01% concentration widely used in immunohistochemistry

APES (3-aminopropyltriethoxysilane)

Very useful in cytology, particularly for cytospin preparations of proteinaceous or bloody material

APES (3-aminopropyltriethoxysilane)

Best section adhesive available and coated slides can be stored for a long time.