Genetics ch 20

These flashcards cover key concepts in molecular technologies and gene cloning discussed in the lecture, emphasizing definitions and functions essential for understanding genetic analysis and principles.

Gene Cloning

The technique of isolating and making many copies of a gene, usually referring to the use of vectors.

Recombinant DNA Technology

The use of in vitro molecular techniques to isolate and manipulate fragments of DNA.

Restriction Enzymes

Enzymes that cut DNA at specific sequences, creating fragments that can be joined with other DNA strands.

Polymerase Chain Reaction (PCR)

A technique used to amplify specific sequences of DNA without the aid of vectors and host cells.

cDNA

Complementary DNA synthesized from an RNA template using the enzyme reverse transcriptase.

CRISPR-Cas Technology

A method for gene editing that allows for the alteration of DNA sequences in living cells.

Northern Blotting

A technique used to identify specific RNA within a mixture of RNA molecules.

Western Blotting

A method used to detect specific proteins in a mixture using antibodies.

Reverse Transcriptase

An enzyme that synthesizes DNA from an RNA template, used in RT-PCR.

Gene Editing

The experimental alteration of a gene sequence, which can be used for gene therapy or research purposes.