Biochemical tests

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Lipid

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4 Terms

1

Lipid

Crush up sample with pestle and mortar, add ethanol to dissolve then water, shake to mix. Positive if milky-white emulsion forms

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2

Proteins

Add Biurets. Positive if changes from blue-black to purple

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3

Reducing sugar

Add Benedict’s solution and heat. Positive if changes from blue to brick red/yellow/green.

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4

Non-reducing sugar

Add Benedict’s solution and heat. Should stay negative. Then boil with acid and neutralise with alkali. Heat with Benedict’s solution. Positive if changes blue to red/orange

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