Biochemical tests

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Last updated 2:27 PM on 5/12/23
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4 Terms

1
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Lipid
Crush up sample with pestle and mortar, add ethanol to dissolve then water, shake to mix. Positive if milky-white emulsion forms
2
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Proteins
Add Biurets. Positive if changes from blue-black to purple
3
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Reducing sugar
Add Benedict’s solution and heat. Positive if changes from blue to brick red/yellow/green.
4
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Non-reducing sugar
Add Benedict’s solution and heat. Should stay negative. Then boil with acid and neutralise with alkali. Heat with Benedict’s solution. Positive if changes blue to red/orange