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Transposon Mutagenesis
Disrupt genes to study function
RB-TnSeq
Barcoded transposon sequencing method
Mutant Fitness
Growth relative to wild type
Gain of Function
Adding gene to study effect
High-Throughput Screening
Large-scale protein/gene analysis
Barcoded Library
Collection of uniquely tagged mutants
RB-TnSeq Goal
Measures mutant fitness using barcodes.
Barcoded Library
Collection of uniquely tagged mutants.
Price et al. (2018)
Studied unknown gene functions.
High-Throughput Analysis
Large-scale protein function analysis.