Lab 9

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disinfectant

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22 Terms

1

disinfectant

used on inanimate objects to lower the levels or inhibit microorganisms growth

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2

antiseptic

used on live tissue to inhibit the growth of microoganism

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3

antimicrobial agent

a natural or synthetic substance that kills or inhibits the growth of microorganisms such as bacteria, fungi and algae

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4

antibiotic

made from another organism to prevent or kill growth in another microorganism

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5

narrow spectrum antibiotic

an antibiotic that is only able to kill or inhibit limited species of bacteria

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6

broad spectrum antibiotic

antibiotics that were effective against both gram-positive and gram-negative bacteria

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7

-cidal

killing

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8

-static

lacking movement, action, or change

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9

a disinfectant must ___ the pathogen while in contact with them

kill

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10

list two types of bacterial cells that are more resistant to disinfectants

gram-negative bacteria: Pseudomonas and K. pneumoniae

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11

why is antimicrobial susceptibility testing done?

to determine which antimicrobials will inhibit the growth of the bacteria of fungi causing specific infection

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12

what is the name of the antimicrobial susceptibility test also known as the filter paper disk agar diffusion method?

Kirby-Bauer test

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13

what is in the filter paper disks?

antibiotics

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14

what kind of agar is used?

Mueller-Hinton

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15

what is streaked onto the agar plate?

organism in question

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16

where are the filter paper disks placed?

on the surface of the agar

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17

what happens to the antimicrobial agent in the disk after it contacts the agar?

diffuse into the agar

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18

after incubation, what does one look for on the plates?

the zone of inhibition

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19

what does "R" mean in interpretation? How is it determined?

Resistance- size of the circle- large circle means it is resistant (zone of inhibition)

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20

What does "S" mean in interpretation? How is it determined?

susceptibility- the size of the circle- small means its susceptible

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21

name a narrow spectrum antibiotic

penicillin

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22

name a broad-spectrum antibiotic

ampicillin

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