MCB 2610 Exam 3 Microbe Minutes

shape of shigella flexneri?

single rods or short chains

what family is S. flexneri under?

enterobacteriaceae

how does S. flexneri get transmitted?

fecal or orally

S. flexneri is the causative agent of?

shigellosis

is S. flexneri a human only pathogen?

yes

dysentery

bloody diarrhea caused by destruction of stomach lining

is S. flexneri more or less susceptible to stomach acid than other bacteria?

less susceptible

does S. flexneri have a low or high infectious dose

low

all 3 species of pathogenic shigella cause?

shigellosis

how many deaths from shigellosis per year?

about 600,000

S. flexneri can be found in?

intestinal epithelial cells

S. flexneri polymerizes actin?

yes

first line of treatment for S. flexneri (shigellosis)

b-lactam antibiotics

S. flexneri is gram-negative or positive?

gram-negative

is S. flexneri a facultative anaerobe?

yes and it has catalase but no oxidase

does S. flexneri form spores or have flagella?

no

optimal growth temp for S. flexneri?

37 C

Haemophilus influenzae is in the __ of __% of healthy adults and children

nasopharynx, 75%

H. influenzae is an __ pathogen

opportunistic

H. influenzae has both __ and __ strains

capsulated and unencapsulated

H. influenzae loves?

heme

H. influenzae is what kind of grower?

fastidious

H. influenzae needs what growth factor?

X (hemin) and V (NAD or NADP)

H. influenzae is usually grown on what kind of medium?

chocolate agar

how is H. influenzae spread?

respiratory droplets

in children under 5 H. influenzae causes?

bacteremia and meningitis

what strain is typed?

capsulated

H. influenzae fatality rate

5-10%

shape of H. influenzae

coccobacilli

is H. influenzae gram-negative or positive

gram-negative

size of H. influenzae

0.3 x 0.8 um

is H. influenzae motile?

no

H. influenzae is a facultative anaerobe, what is it's optimal growth temp?

37 C

H. influenzae is known as capnophilic meaning?

it can withstand higher levels of CO2 for growth

agrobacterium tumefaciens shape

rod-shaped with peritrichous flagella

A. tumefaciens is a __ pathogen

plant

what family is A. tumefaciens under?

Rhizobiaceae

A. tumefaciens is a __ fixing bacteria

nitrogen

A. tumefaciens genome

linear and circular chromosome and 2 plasmids

what plasmid does A. tumefaciens contain for pathogenicity

Ti plasmid

A. tumefaciens does chemotaxis towards?

phenolic compounds

A. tumefaciens carries __ __ genes important in formation of t-pilus and encodes virulence genes necessary for __ formation

25 virulence, tumor

why does A. tumefaciens form a t-pilus?

to move tDNA into plant

is A. tumefaciens gram-negative or positive?

gram-negative

A. tumefaciens is aerobic and forms?

biofilms

A. tumefaciens optimal growth?

28 C

size of A. tumefaciens?

1.5-3.0 um x 0.6-1.0 um

Francisella tularensis is motile?

no

F. tularensis is found in?

deer, squirrel, rabbits, birds and reptiles

tularemia

rabbit or deer fly fever (100-200 cases/year)

how is F. tularensis transferred to humans?

from animals and insects, no human to human

F. tularensis is a tier 1 agent meaning?

poses severe potential threat to public health

F. tularensis can be aerosolized leading to its most dangerous form, this can be bad because?

it has a low infectious dose

F. tularensis requires __ for growth

cysteine

F. tularensis - 5 types of infection

skin exposure, eye, inhalation, ingestion and systemic infection

F. tularensis - skin exposure

glandular and ulceroglandular, can cause skin infections

F. tularensis - inhalation

pneumonic, most deadly/serious

F. tularensis - eye

oculoglandular

F. tularensis - ingestion

oropharyngeal

F. tularensis - systemic infection

typhoidal - can be deadly

F. tularensis is gram-negative or positive?

gram-negative

shape of F. tularensis

coccobacillus

F. tularensis is aerobic, what's its optimal growth and does it form spores?

37 C, no spores

size of F. tularensis

0.2-0.7 um x 0.2 um

H. influenzae has both __ and __ strains

is S. flexneri a facultative anaerobe?

first line of treatment for S. flexneri (shigellosis)

b-lactam antibiotics

S. flexneri can be found in?

intestinal epithelial cells

how does S. flexneri get transmitted?

fecal or orally

S. flexneri is the causative agent of?

shigellosis

is S. flexneri a human only pathogen?

yes

dysentery

Pectobacterium carotovorum is one of the most destructive?

plant pathoges

P. carotovorum is under what family?

enterobacteriaceae

P. carotovorum is an __ pathogen

opportunistic

P. carotovorum causes __ __ __ diseases of plants

bacterial soft rot

P. carotovorum contains __ enzymes

pectolytic

P. carotovorum infects?

fruit/plant itself + root

P. carotovorum has what kind of flagella?

peritrichous

P. carotovorum is transmitted via?

contaminated irrigation water/degrading plant material

P. carotovorum is gram-negative or positive

gram-negative

P. carotovorum is facultative anaerobe and forms what shapes?

singlets or rod

size of P. carotovorum

0.5-1.0 um x 1.0-3.0 um

optimal growth temperature for P. carotovorum

20-25 C