MLSCI 340 QC Quiz

Staphylococcus sp catalase

+

Staphylococcus aureus slide coagulase

+

CNS Staph slide coagulase

-

Staphylococcus aureus tube coagulase

+/- after 4 hours

+/- after 24 hours

CNS staph tube coagulase

-

Staphylococcus lugdunensis slide coagulase

variable

Staphylococcus intermedius slide coagulase

variable

Micrococcus coagulase

Slide and tube neg

Rothia mucilaginosa

- / wk+

Staphylococcus aureus RapidStaph results

+

CNS staph Rapid Staph results

-

Staphylococcus bacitracin results

Resistant

Miccrococcus sp. bacitracin results

Susceptible (>10mm)

What organism could be a positive control for Novobiocin

S.saprophyticus

What organism(s) can be a negative control for novobiocin

S. epidermidis, S.lugdunensis, S.intermedius (all susceptible)

Positive control organism for Cefoxitin (resistant)

MRSA

Negative control organism for Cefoxitin (susceptible)

S.aureus

Staphylococcus aureus color on a SASelect

Pk

S.epidermidis color on SASelect

Wt

S.saprophyticus color on SASelect

Blue

S.lugdunensis color on SASelect

Wt

S.intermedius color on SASelect

Blue-grey

What could be a negative control for denim blue chromagar

S.aureus (no growth)

What could be a positive control denim blue chromagar

MRSA (blue colonies)

Negative control for catalase

Any Streptococci. Often S.pyogenes

What organisms could be weak pos controls for catalase

E.faecalis, A.urinae

S.pyogenes PYR result

+

S.agalactiae PYR result

-

Group C/G Strep PYR result

-

S.anginosus PYR result

-

Enterococcus faecalis PYR result

+

S.bovis PYR result

-

A.urinae PYR result

-

Latex group of S.pyogenes

A

Latex group of S.agalactiae

B

S.anginosus Latex group

F (may show as A, C, G)

What could be a positive control of CAMP test

S.agalactiae

What could be a negative control of CAMP test

S.pyogenes, Group C/G Strep, S.anginosus

S.pyogenes Bacitracin result

S

S.agalactiae bacitracin result

R

Group C/G strep bacitracin result

R

S.anginosus bacitracin result

R

S.pneumoniae Optochin result

S

Viridans group strep optochinr result

R

S.pneumoniae bile salt result

+

Viridans group strep Bile salt result

-

E.faecalis bile esculin result

+

S.bovis bile esculin

+

Aerococcus urinae bile esculin result

-

E.faecalis 6.5% NaCl

+

A.urinae 6.5% NaCl

+

S.bovis 6.5% NaCl

-

Oxidase result of Neisseria sp.

+

Oxidase of M.catarrhalis

+

Neisseria sp. Butyarate hydrolysis

-

M.catarrhalis Butyrate hydrolysis

+

Positive control for Thayer martin growth

N. gonorrhoeae or N.meningitidis

N.gonorrhoeae CTA sugars

Dextrose Maltose Sucrose Lactose

+ - - -

N.meningitidis CTA sugars

Dextrose Maltose Sucrose Lactose

+ + - -

N.lactamica CTA sugars

Dextrose Maltose Sucrose Lactose

+ + - +

N.mucosa / poly CTA sugars

Dextrose Maltose Sucrose Lactose

+ + + +

M.catarrhalis CTA sugars

Dextrose Maltose Sucrose Lactose

- - - -

B.cereus catalase

+

Lactobacillus sp. catalase

-

L.monocytogenes catalase

+

L.monocytogenes BEA

+

L.monocytogenes TSI

A/A (sick yellow)

L.monocytogenes TSB motlity @ RT

+ tumbling

L.monocytogenes TSB motility at 37C

-

Erysipelothrix sp. catalase

-

Erysipelothrix sp. BEA

-

Erysipelothrix sp. TSI

A/A H2S (weak, long time)

C.diphtheriae catalase

+ (strong)

C.diphtheriae CTBA

Black colonies

Diphtheroids catalase

+

Diptheroids CTBA

ng or growth without blackening

What is CTBA

Cysteine tellurite blood agar

Arcanobacterium hemolyticum catalase

-

Arcanobacterium hemolyticum CAMP inhibition

+

H.influenzae growth requirements

XV X V

+ - -

Requires both X and V

H.influenzae ALA

-

H.parainfluenzae growth requirements

XV X V

+ - +

Requires V only

H.parainfluenzae ALA

+

Pasteurella multocida oxidase

+

Pasteurella multocida catalase

+

Pasteurella multocida TSI

A/A sick yellow

Pasteurella multocida indole

+

Bordetella pertussis oxidase

+

Bordetella pertussis catalase

+

Candida albicans germ tube

+

Candida tropicalis germ tube

-

Candida krusei germ tube

-

Candida albicans chromogenic candida

green

Candida tropicalis chromogenic candida

blue

Candida krusei chromogenic canddia

dry purple

What enterics are pink on a MAC

E.coli, Klebsiella sp, Enterbacter sp, Citrobacter sp

LESerratio marcescens MAC

red

What enterics are white on MAC

Proteus sp. Providencia rettgeri, Morganella morganii, Salmonella sp., Shigella sp., Yersinia entercolitica

Lactose fermenters TSI result

A/A g (Citrobacter has H2S)

Citrobacter koseri TSI

K/A g