IRONMAN 1

Infiltration: celloidin

Embedding: paraffin

Double embedding involves

Picric acid

Used to remove formalin pigments

Iodine

Used to remove mercurial deposits

Picric acid

Explosive when dry

Mordant

Function of alum in hematoxylin

Rapid diagnosis

Primary importance of frozen sections

Frozen section

Tissue examination method for enzyme histochemistry

Phase contrast microscopy

Second best choice for routine cytologic examination after Papanicolau

Bouin's

Fixative NOT SUITABLE for kidney structures

COAGULATION NECROSIS

Cell death due to ischemia (loss of blood supply) is known as infarction, and is manifested by this characteristic histologic appearance

Clostridium difficile

Pseudomembranous colitis and diarrhea

Corynebacterium amycolatum

Most frequently recovered Corynebacterium species from human clinical material. It is part of the normal skin microbiota

Candida albicans

Primary fungal pathogen in HIV patients

Acanthamoeba castellanii

Doublewalled, wrinkled cyst form

Leishmanial form

Intracellular form of blood and tissue flagellates

pH 7 to 8

Normal stool pH

pH 5.5 or less

Stool pH associated with CHO disorders

10,000 g for 5 minutes

Centri speed and time for microhematocrit

Creamy layer: chylomicrons

Turbid: VLDL

Standing plasma test for lipids

Osmic acid/osmium tetroxide

- it inhibits hematoxylin

Microanatomical fixatives should never contain

Glacial acetic acid

- has affinity for nuclear chromatin

Nuclear fixatives should contain

Glacial acetic acid

- destroys mitochondria and Golgi bodies

Cytoplasmic fixatives should never contain

Paldwell Treffall

Cambridge/Rocking microtome was invented by

Coulombic attraction/electrostatic bonds, hydrogen bonds

Bond between Best carmine and glycogen

Regressive staining

- involves a differentiation step

Staining method of routine H&E

PAS

Azocarmine

Stains for the glomerular basement membrane

Immediately after death, rubbery consistency

Postmortem clotting

Friable, characterized by fibrin precipitation

Antemortem thrombi

Cryoprecipitate (fibrinogen)

Topical thrombin

Components of fibrin glue

2 wks

Donor deferral, measles (rubeola) vaccination

4 wks

Donor deferral, German measles (Rubella) vaccination

Glitter cells: light blue

Neutrophils: violet

Color of glitter cells and neutrophils under Sternheimer-Malbin stain

Prussian blue

Stain for hemosiderin granules in urine

Prealbumin (transthyretin)

Second most prevalent protein in CSF

Fetal distress

Meconium may be present in amniotic fluid as a result of

Nephrotoxicity

Ototoxicity

Adverse reaction to Aminoglycosides

TETANY

Neuromotor irritability accompanied by muscular twitching and eventual convulsions; generally due to low calcium levels (hypocalcemia)

Hypocalcemia

Tetany is generally due to

1% NaOH

Reagent for the APT test

Fetal blood: pink soln

Maternal blood: yellow-brown supernatant

APT test result for fetal and maternal blood

Florence test

- Iodine, KI/ dark brown rhombic crystals

Test for choline

Barbiero's test

- Picric acid, TCA/ yellow leafshaped crystals, needles

Test for spermine

Blondheim's test

- ammonium sulfate will precipitate hemoglobin

Test to differentiate hemoglobin from myoglobin in urine

Plastic

Embedding medium for EM

Neutral red

Best vital stain

Janus Green

Vital stain for mitochondria

Early pregnancy

Ferning is seen during

Harris hematoxylin

OG 6

EA

Stains in Pap's

1200 mL/min

Total renal BLOOD flow

600 to 700 mL/min

Total renal PLASMA flow