HISTOPATH (FIXATION) (METALLIC FIXATIVES)

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Metallic chloride

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Most common metallic fixative

Secondary fixative

May contain black precipitates

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Zenker’s Fluid

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Good general fixative

Excellent staining results

Liver, spleen, connective tissue fiber, and nuclei Trichome staining

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9 Terms

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Metallic chloride

Most common metallic fixative

Secondary fixative

May contain black precipitates

2
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Zenker’s Fluid

Good general fixative

Excellent staining results

Liver, spleen, connective tissue fiber, and nuclei Trichome staining

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Helly’s Solution

Zenker’s Formol also known as:

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Zenker’s Formol

Microanatomic fixative for pituitary gland, bone marrow, and blood-containing organs (Spleen and liver) Preserves cytoplasmic granules well

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12-24 hours

Zenker’s Formol (Duration)

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Heidenhan’s Susa Solution

Tumor biopsies and excellent cytologic fixative

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3-12 hours

Heidenhan’s Susa Solution (DURATION)

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B5 Fixative

Bone marrow biopsies

Very fast-acting fixative

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1.5 - 2 hours

B5 Fixative (DURATION)