Core Summary Sheet-(Spectroscopy Lab, Quinine Fluorescence Lab, FTIR Infrared Lab)

These flashcards cover key concepts and terms related to the spectroscopy labs, including instrument components, methods, data acquisition, and analysis.

What is the function of the Xenon flash lamp in the Cary 60 UV-Vis Spectroscopy Lab?

It provides a broad UV-Vis range of light for analysis.

What is the purpose of the monochromator in spectroscopy?

It selects a single wavelength (λ) for analysis.

What type of lamp does the Eclipse Fluorimeter use?

It uses a Xenon arc lamp as the UV excitation source.

How is fluorescence emission detected in the Quinine Fluorescence Lab?

Using a PMT (Photomultiplier Tube) detector at a 90° angle.

What is the range of scans selected in the FTIR Infrared Lab?

The range is from 4000 to 400 cm⁻¹.

What is the first step in setting up the Spectroscopy Lab experiment?

Set λmax for the compound.

What do you do to prepare standards in the Quinine Fluorescence Lab?

Prepare standards by serial dilution of the stock solution.

In the FTIR lab, what is the purpose of applying pressure to the sample?

To obtain a better spectrum by ensuring good contact with the ATR crystal or KBr cell.

What method do you use to find unknown concentrations in the Spectroscopy Lab?

Use linear regression on the plotted A vs concentration curve.

What formula is used to relate absorbance to concentrations in spectroscopy?

A = εcl (Beer's Law).