General Biology 1 Lab: 8

Write the general equation for photosynthesis

6Co2+12H2O-Light→ C6H12O6 + O2 + 6H2O

Explain the demonstration showing the necessity of light for photosynthesis

necessary for the light- dependent reaction

Light is absorbed by the chlorophyll and water to produce
- NADPH, ATP, H+, Oxygen, and Water

What does ATP and NADPH allow for in the light dependent Phase

Allows for carbon dioxide to be reduced into glucose

Demonstrate the Test for starch for light and co2

• Iodine is used to test for starch

• will turn purple for starch

• Brown for no starch

Explain the demonstration showing the necessity of carbon dioxide for photosynthesis

Co2 in photosynthesis allows for it to be reduced into Glucose

starch

State the pigments found in the Coleus Leaf

Chlorophyll- Green

Anthocyanins- purple

Carotenoids- Yellow

State the order of color from outside to inside

Green(Chlorophyll) , white, pink only(anthocyanins only), red/purple (Anthocyanins+Chlorophyll)

What was the index card used for

• Used to see the effect of light on photosynthesis

• light reaction/ Investing light

• The open part was green, the covered part was brown
  

What was petroleum jelly used for

Used to see gas/CO2 exchange
Covered with jelly was lighter, Uncovered was darker/ purple with iodine test
The normal side was green, and the side covered with jelly was green/slightly yellow.

Describe what each does
boiling water

boiling alcholling

Boiling water- Breaks cell wall

boiling alcohol- Removes pigment

What is the purpose of chromatography solvent

pigments dissolve in chromatography solvent, which allows them to be separated

• pigmentation dissolves which allows them to be separated

What is the purpose of using extract of chloroplasts

Chloroplasts contain photosynthetic pigment (chlorophyll)

From top to bottom Explain the orders of pigments

Carotenes- orange

xanthophylls- yellow

chlorophyll a- green

chlorophyll b- green

Why does chlorophyll b appear yellow and green

It reflects yellow and green whilst it absorbs red and purple

Why did the different photosynthetic pigment migrate to different point on the chromatography paper

The pigments differ in their solubility in the solvent and affinity for the paper.