Isolation of Unusual or Fastidious Bacteria
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8 Terms
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Legionella pneumophila
Buffered charcoal yeast extract (BCYE) agar with Lcysteine
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Francisella tularensis
Cystine heart agar with 9% chocolatized sheep blood
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Bordetella pertussis
Bordet-Gengou
Regan-Lowe medium
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Brucella spp.
Extended incubation (≥7 days),
enriched blood culture media
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Mycobacterium tuberculosis
Requires Lowenstein-Jensen medium;
may take 3–6 weeks to grow
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Leptospira spp.
Requires Fletcher’s or EMJH medium with extended incubation (up to 6 weeks)
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Nocardia spp.
May require selective media and prolonged incubation;
often mistaken for contaminants
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Anaerobes
e.g. Bacteroides fragilis
Require prereduced anaerobically sterilized (PRAS) media,
anaerobic jars/chambers
Note 
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