Isolation of Unusual or Fastidious Bacteria
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8 Terms
Legionella pneumophila
Buffered charcoal yeast extract (BCYE) agar with Lcysteine
Francisella tularensis
Cystine heart agar with 9% chocolatized sheep blood
Bordetella pertussis
Bordet-Gengou
Regan-Lowe medium
Brucella spp.
Extended incubation (≥7 days),
enriched blood culture media
Mycobacterium tuberculosis
Requires Lowenstein-Jensen medium;
may take 3–6 weeks to grow
Leptospira spp.
Requires Fletcher’s or EMJH medium with extended incubation (up to 6 weeks)
Nocardia spp.
May require selective media and prolonged incubation;
often mistaken for contaminants
Anaerobes
e.g. Bacteroides fragilis
Require prereduced anaerobically sterilized (PRAS) media,
anaerobic jars/chambers
Note 
Flashcards (33)