Micro Lab Final - UV light
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5 Terms
1
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What kind of radiation is UV light
non-ionizing radiation
2
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Explain the molecular basis for the effect of UV light on the growth of the bacteria
* UV light damages cellular DNA → formation of dimers between adjacent pyrimidine bases
* leads to lethal consequences for unicellular organisms
* If DNA is not in the proper form → DNA polymerase can not transcribe to make mRNA→ translation will not occur and proteins will not be made
* without proteins that cell can not carry out metabolic functions and growth can not occur
* leads to lethal consequences for unicellular organisms
* If DNA is not in the proper form → DNA polymerase can not transcribe to make mRNA→ translation will not occur and proteins will not be made
* without proteins that cell can not carry out metabolic functions and growth can not occur
3
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Is UV light penetrating
non-penetration
* if bacteria is covered it will not work (ex. capsule)
* mostly used to kill airborne or surface microbes
* if bacteria is covered it will not work (ex. capsule)
* mostly used to kill airborne or surface microbes
4
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Predict what will happen when bacteria is exposed to UV light
the longer the UV light the less growth
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What is the effect of UV light on endospore formation
* endospore formation will lead to resistance of UV light
* the endospore must be formed before UV light exposure, because it takes time to form the spore
* ex. growing bacteria under harsh conditions → spore formation and then placing under UV light
* the endospore must be formed before UV light exposure, because it takes time to form the spore
* ex. growing bacteria under harsh conditions → spore formation and then placing under UV light