Culture Media

In motility test agar, motile bacteria show growth away from what?

stab line

In motility test agar, nonmotile bacteria remain where?

stab line

On CIN, mannitol fermenters form what characteristic colony pattern?

bullseye

On CIN, nonmannitol fermenters appear as what type of colonies?

translucent

On EMB, nonlactose fermenters appear as what type of colonies?

translucent

On EMB, what colony appearance suggests lactose fermentation?

purple-black

On EMB, what sheen is associated with strong lactose fermentation?

green

On HEA, H2S-positive organisms show what color change?

black

On HEA, lactose or sucrose fermenters appear what color?

yellow

On HEA, nonlactose or nonsucrose fermenters appear what type of colonies?

translucent

On MacConkey agar, lactose fermenters appear what color?

pink

On MacConkey agar, nonlactose fermenters appear what type of colony?

translucent

On SSA, H2S-positive organisms show what color change?

black

On SSA, lactose fermenters appear what color?

pink

On SSA, nonlactose fermenters appear what type of colonies?

translucent

On TCBS, sucrose fermenters appear what color?

yellow

On TCBS, sucrose nonfermenters appear what color?

green

Which differential medium distinguishes mannitol-fermenting Yersinia by bull's-eye colonies?

CIN

Which differential medium distinguishes sucrose-fermenting and sucrose-nonfermenting Vibrios?

TCBS

Which differential medium gives lactose fermenters purple-black colonies with a green metallic sheen?

EMB

Which differential medium separates lactose fermenters from nonlactose fermenters among enterics and gives pink or red LF colonies?

MAC

Which differential medium uses motility away from a stab line as its key readout?

MTA

Which medium differentiates lactose and sucrose fermentation plus H2S production among enterics?

HEA

Which medium differentiates lactose fermentation and H2S production and may also be selective for Salmonella/Shigella?

SSA

Which medium differentiates motile from nonmotile bacteria by growth away from the stab line?

MTA

Which medium shows bull's-eye colonies for mannitol-fermenting Yersinia?

CIN

Which medium shows pink or red lactose-fermenting colonies?

MAC

Which medium shows purple-black colonies with metallic sheen for lactose fermenters?

EMB

Which medium shows yellow colonies for sucrose-fermenting Vibrios?

TCBS

CNA supports what broad organism group according to the notes?

gram positive

Dyes, alcohols, acids, and antimicrobials are used in what media type?

selective

Five percent sheep blood is an example additive in what media type?

enriched

MacConkey agar can act as which two media types?

selective, differential

TSA is an example of what media type?

nonselective

Vitamins are typical additives in what media type?

enriched

Which media type adequately supports growth of most organisms?

nonselective

Which media type both supports growth and distinguishes colony behavior?

differential

Which media type commonly contains growth enhancers?

enriched

Which media type commonly uses inhibitory dyes or acids?

selective

Which media type contains growth enhancers such as sheep blood and vitamins?

enriched

Which media type supports growth and also differentiates within the group?

differential

Which media type uses inhibitory substances to select a group of organisms?

selective

In thioglycolate broth, oxygen tension decreases toward what part of the tube?

bottom

What component in BCYE helps bind and sequester growth inhibitors?

charcoal

Which Haemophilus species exception is listed for sheep blood agar?

Haemophilus

Which agar is abbreviated SBA in microbiology notes?

sheep blood

Which broth is used for cultivation of bacteria including microaerophilic and obligate anaerobes?

thioglycolate

Which broth supports microaerophilic and obligate anaerobic growth?

thioglycolate

Which genus is an exception listed for sheep blood agar along with Neisseria and Haemophilus?

Legionella

Which medium contains cysteine and iron supplementation for Legionella recovery?

BCYE

Which medium is best associated with Legionella recovery in the notes?

BCYE

Which medium is best associated with fastidious Neisseria and Haemophilus isolation?

chocolate agar

Which medium is standard for many common bacterial AST procedures?

Mueller-Hinton

Which nonselective medium is used for antimicrobial susceptibility testing of many common bacteria?

Mueller-Hinton

Which nonselective medium is used for cultivation and isolation of fastidious bacteria?

chocolate agar

Which nonselective medium is used for recovery of Legionella species?

BCYE

Which nonselective medium supports the growth of most bacteria?

SBA

Which organisms are exceptions that do not grow well on sheep blood agar according to the notes?

Neisseria

ANC agar is designed mainly for recovery of which bacterial shape group?

streptococci

Hektoen enteric agar uses which dye listed second with acid fuchsin?

acid fuchsin

Hektoen enteric agar uses which pH indicator dye listed first?

bromthymol blue

LIM broth is designed mainly for recovery of which streptococcus species?

agalactiae

MacConkey agar uses which inhibitor pair?

crystal violet

Regan-Lowe medium is designed mainly for recovery of which genus?

Bordetella

SSA uses which inhibitor besides bile salts and brilliant green?

sodium citrate

TCBS is designed mainly for recovery of which genus?

Vibrio

What is the basis of selectivity for CIN agar in the notes besides antimicrobials?

crystal violet

What is the basis of selectivity for EMB?

aniline dyes

What is the basis of selectivity for MacConkey agar?

bile salts

What is the basis of selectivity for Selenite broth?

selenite

What is the basis of selectivity for Thayer-Martin medium?

antimicrobials

Which broth is used for recovery of S. agalactiae?

LIM

Which enrichment broth uses sodium selenite for Salmonella recovery?

Selenite

Which medium is abbreviated CIN?

CIN

Which medium is abbreviated HEA?

HEA

Which medium is abbreviated TCBS?

TCBS

Which selective medium contains vancomycin, colistin, nystatin, and SXT?

Thayer-Martin

Which selective medium enhances recovery of Salmonella and Shigella?

HEA

Which selective medium is used for recovery of Vibrio species?

TCBS

Which selective medium recovers Bordetella pertussis and Bordetella parapertussis?

Regan-Lowe

Which selective medium recovers Campylobacter species?

Campy BAP

Which selective medium recovers Neisseria species from nonsterile sites?

Thayer-Martin

Which selective medium recovers Salmonella and Shigella but may inhibit Shigella and is not recommended for primary isolation?

SSA

Which selective medium recovers Yersinia species?

CIN

Which selective medium recovers anaerobic streptococci?

ANC

Which selective medium uses aniline dyes to recover hardy enteric gram-negative rods?

EMB

Which selective medium uses antimicrobials and crystal violet for Yersinia recovery?

CIN

Which selective medium uses antimicrobials for anaerobic streptococci recovery?

ANC

Which selective medium uses bile salts and crystal violet to recover hardy enteric gram-negative rods?

MacConkey

Which selective medium uses bile salts and low pH to inhibit most enterics while recovering Vibrio?

TCBS

Which selective medium uses bile salts, bromthymol blue, and acid fuchsin?

HEA

Which selective medium uses bile salts, sodium citrate, and brilliant green?

SSA

Which selective medium uses cephalothin, vancomycin, trimethoprim, amphotericin B, and polymyxin B?

Campy BAP